Role of ultrasound and l-lysine/l-argnine in improving the physical stability of myosin-soybean oil emulsion

• Published in: Food hydrocolloids Vol. 111; p. 106367
• Main Authors: Huang, Yajun, Zhang, Daojing, Zhang, Yinyin, Fang, Hongmei, Zhou, Cunliu
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-02-2021
• Subjects: Emulsifying property; L-argnine; L-lysine; Myosin-soybean oil emulsions (MSE); Structure; Ultrasound; Emulsifying property; Myosin-soybean oil emulsions (MSE); Structure; Ultrasound; L-argnine; L-lysine
• ISSN: 0268-005X; 1873-7137
• DOI: 10.1016/j.foodhyd.2020.106367

This work investigated the mechanisms underlying the combined effects of ultrasound and l-lysine/l-arginine on the physical stability of myosin-soybean oil emulsions (MSE). The results showed that compared with the individual treatments (ultrasound, l-lysine or l-arginine), the combined treatments (ultrasound and l-lysine, ultrasound and l-arginine) were more effective in decreasing creaming index and emulsion size, but increasing emulsifying activity index, emulsifying stability index and emulsion ζ-potential, and forming smaller and more uniform MSEs. Also, except that active sulfhydryls decreased in the following order: ultrasonic treatment > a combination of Arg and ultrasound ≈ a combination of Lys and ultrasound > Arg ≈ Lys ≈ control, the combined treatments were effective than the individual treatments in increasing the ζ-potential, surface hydrophobicities, and solubility of myosin as well as the percentage of adsorbed myosin, but decreasing the hydrodynamic size of myosin, and inducing the transformations of myosin from a rigid structure to flexible ones. Therefore, the results suggested that ultrasound and l-lysine/l-arginine synergistically improved the physical stability of MSEs by inducing myosin to unfold and be negatively charged.
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•Ultrasound and lysine/arginine were used to stabilize myosin-soybean oil emulsion.•Combined treatment more effectively raised emulsion stability than single treatment.•Combined treatment more effectively raised myosin ζ-potential than single treatment.•Combined treatment more effectively made myosin be flexible than single treatment.