Enhancing polysaccharide production by Paraisaria dubia spores batch fermentation through a pH-shift strategy based on kinetic analysis

• Published in: Process biochemistry (1991) Vol. 126; pp. 292 - 298
• Main Authors: Yuan, Li, Tong, Ling-Ling, Wang, Yue, Du, Yuan-Hang, Liu, Meng-Zhen, He, Shao-Jie, Wei, Shi-Xiang, Zhang, Yi-Dan, Chen, Zi-Lei, Jin, Su-Xing, Guo, Dong-Sheng
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2023
• Subjects: Cordyceps polysaccharide; Fermentation optimization; Kinetic analysis; Paraisaria dubia; pH-shift strategy; Spores fermentation; pH-shift strategy; Kinetic analysis; Spores fermentation; Fermentation optimization; Cordyceps polysaccharide; Paraisaria dubia
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2022.12.020

This study aims to develop a fermentation method instead of fruiting body extraction for Cordyceps polysaccharide (CP). The fermentation characteristics of P. dubia at different pH were inspected. The mechanism of microcycle conidiation of P. dubia was activated under pH 9.0 and produced yeast-like spores. A pH-shift strategy was raised based on the analysis of fermentation kinetics parameters. And, yeast-like spores were used as fermentation seeds to increase culture density. To further improve efficiency, a fed-batch fermentation with a pH-shift strategy was executed, CP production reached 13.54 g/L, with the productivity of 0.42 g/(L d), and the specific rate of CP to biomass was increased to 0.27, which improved by 47.6 %, 64.3 % and 97.1 % respectively, compared with fermentation without process controls. And, CP from fermented and extracted had the same monosaccharide composition except for differences in molecular weight. This study provides a feasible strategy for the efficient preparation of CP through spores fermentation.
[Display omitted]
•Microcycle conidiation of P. dubia was activated at pH 9.0 and produced spores.•A spores fermentation method was first introduced to the production of CP.•The stage kinetic characteristics of CP fermentation by P. dubia were proposed.•A maximum CP yield of 13.54 g/L was gained in fed-batch with pH-shift strategy.