Short daylight photoperiod alleviated alarm substance-stimulated fear response of zebrafish

• Published in: General and comparative endocrinology Vol. 338; p. 114274
• Main Authors: Tan, Mei-Ling, Xie, Cheng-Ting, Tu, Xin, Li, Ying-Wen, Chen, Qi-Liang, Shen, Yan-Jun, Liu, Zhi–Hao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2023
• Subjects: Animals; Cortisol; Dopamine - metabolism; Fear; Fear response; Female; Male; Melatonin; Melatonin - metabolism; Neurotransmitters; Photoperiod; Serotonin; Zebrafish; Zebrafish - metabolism; HPI; Melatonin; VMAT; ACTH; Cortisol; COMT; TPH; SD; AANAT; Fear response; 5-HT1A/1B; MT; qRT-PCR; Zebrafish; Photoperiod; Neurotransmitters; SERT; TH; DAT; LD; CRH; MAO; 5-HT; DA; CRHR1/CRHR2; DrD1/DrD2
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2023.114274

•Long photoperiod failed to affect AS-stimulated fear response in zebrafish.•Short photoperiod alleviated AS-stimulated fear response in zebrafish.•Short photoperiod alleviated fear response through MT/5-HT/DA pathways and HPI axis.
Photoperiod has been well-documented to be involved in regulating many activities of animals. However, whether photoperiod takes part in mood control, such as fear response in fish and the underlying mode(s) of action remain unclear. In this study, adult zebrafish males and females (Danio rerio) were exposed to different photoperiods, Blank (12 h light: 12 h dark), Control (12 h light: 12 h dark), Short daylight (SD, 6 h light: 18 h dark) and Long daylight (LD, 18 h light: 6 h dark) for 28 days. After exposure, fear response of the fish was investigated using a novel tank diving test. After alarm substance administration, the onset to higher half, total duration in lower half and duration of freezing in SD-fish were significantly decreased, suggesting that short daylight photoperiod is capable of alleviating fear response in zebrafish. In contrast, comparing with the Control, LD didn’t show significant effect on fear response of the fish. Further investigation revealed that SD increased the levels of melatonin (MT), serotonin (5-HT) and dopamine (DA) in the brain while decreased the plasma level of cortisol comparing to the Control. Moreover, the expressions of genes in MT, 5-HT and DA pathways and HPI axis were also altered consistently. Our data indicated that short daylight photoperiod might alleviate fear response of zebrafish probably through interfering with MT/5-HT/DA pathways and HPI axis.