Chlorella sp. MBFJNU-17 as an emerging source of starch: Changes in starch structures during heterotrophic cultivation

• Published in: Industrial crops and products Vol. 204; p. 117345
• Main Authors: Chi, Chengdeng, Zhou, Youcai, Cheng, Feiyang, Guo, Xu, Chen, Bilian, Zhang, Yiping, He, Yongjin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-11-2023
• Subjects: Heterotrophic cultivation; Microalgae starch; Starch source; Starch structure; Microalgae starch; Heterotrophic cultivation; Starch structure; Starch source
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2023.117345

Chlorella sp. MBFJNU-17 rich in starch and protein could be obtained by modulating the glucose and urea content during its cultivation. Whether MBFJNU-17 was a potential source of starch with adjustable structures was still unclear. In this study, MBFJNU-17 cells with different starch content were collected by regulating glucose (3–15 g/L) and urea content (0–4 g/L) during heterotrophic cultivation. Cells cultured with high glucose content (15 g/L) and low urea content (0 g/L) had a high starch content. Moreover, the starches produced by these cells displayed a larger molar mass and a higher proportion of ordered structures. On the other hand, cells cultured with limited glucose content (3 g/L) and sufficient urea content (0 g/L) showed lower starch content, along with lower molar mass and a reduced amount of ordered structures. Starch granules derived from cells with higher glucose culture conditions demonstrated lower digestibility. Findings suggested that MBFJNU-17 cells with adjustable starch structures and digestibility could be obtained by modulating heterotrophic cultivation conditions.
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•Starches were isolated from MBFJNU-17 cells with different cultivations.•The cultivation conditions notably affected starch structures and digestibility.•Both starch structures and protein content affected starch digestibility.