Reproductive analysis of sexual interference and role of pollinators in sunnhemp: a late-acting self-incompatible crop

• Published in: Euphytica Vol. 219; no. 10; p. 112
• Main Authors: Maruthi, Rangappa Thimmaiah, Kumar, Arroju Anil, Choudhary, Shashi Bhushan, Sharma, Hariom Kumar, Prasannakumar, N. R., Thribhuvan, R., Saha, Dipnarayan, Mitra, Jiban
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2023; Springer; Springer Nature B.V
• Subjects: Abscission; Analysis; Animal reproduction; Biomedical and Life Sciences; Biotechnology; Breeding success; Crops; Flowers; Hermaphrodites; Incompatibility; Interference; Life Sciences; Longevity; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Pollen; Pollination; Pollinators; Reproduction; Reproductive behavior; Seed set; Self-incompatibility; Sexual behavior; Vegetable fibers; Bast fibre crop; sp; Breeding system; Pollinator behaviour
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-023-03243-9

Sunnhemp (
Crotalaria juncea
), is a commercially important bast fibre crop that possesses late-acting self-incompatibility. Crops with hermaphrodite flowers expressing late-acting self-incompatibility like sunnhemp often encounter sexual interference. The impact of sexual interference on reproductive output in sunnhemp can be studied by understanding its reproductive mechanism and pollinator behaviour. Here, we reported the consequence of different possible types of pollination in sunnhemp under natural conditions and the role of pollinator behaviour in sexual interference. Controlled hand-pollination experiments and seed set analysis were done to understand the influence of sexual interference on seed setting. Self-pollination and pistil longevity studies were undertaken to determine the effect of self-pollination on pistil abscission. Through field observations pollinator identity and how they interact with flowers were determined. Pollination studies highlight the importance of pollen quality over quantity in determining the reproductive success of sunnhemp. The differential pistil longevity between unpollinated and self-pollinated flowers reassured the presence of late-acting self-incompatible in sunnhemp.
Xylocopa auripennis
,
Xylocopa fenestrate
and
Xylocopa ruficornis
were the most effective pollinators. We conclude that under natural conditions there was sexual interference through self-pollination and geitonogamy, which often causes pollen limitation and ovule discounting in sunnhemp.