Structural, antioxidant, prebiotic and anti-inflammatory properties of pectic oligosaccharides hydrolyzed from okra pectin by Fenton reaction

• Published in: Food hydrocolloids Vol. 118; p. 106779
• Main Authors: Yeung, Yuen Kwan, Kang, Yu-Ra, So, Bo Ram, Jung, Sung Keun, Chang, Yoon Hyuk
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-09-2021
• Subjects: Anti-inflammatory activity; Antioxidant activity; Fenton reaction; Pectic oligosaccharide; Prebiotic activity; Pectic oligosaccharide; Fenton reaction; Anti-inflammatory activity; Prebiotic activity; Antioxidant activity
• ISSN: 0268-005X; 1873-7137
• DOI: 10.1016/j.foodhyd.2021.106779

To elucidate optimum conditions for the production of pectic oligosaccharides (POSs) with excellent antioxidant, prebiotic, and anti-inflammatory activities, okra pectin (OP) was hydrolyzed by Fenton reaction with different concentrations (3 mM, 5 mM, and 7 mM) of FeSO4. Molecular weight of OP was 112.31 kDa. After Fenton reaction with 3 mM, 5 mM, and 7 mM of FeSO4 (products were named as POS3, POS5, and POS7, respectively), molecular weights were significantly reduced to 6.09 kDa, 4.89 kDa, and 1.79 kDa, respectively. This indicates that Fenton reaction is an efficient way to hydrolyze pectin to obtain POSs. Regarding monosaccharide compositions, FT-IR spectra, and NMR spectra ascertained that ·OH produced by Fenton reaction attacked the homogalacturonan region and neutral side chains of rhamnogalacturonan-I. POSs had higher antioxidant activities (DPPH and ABTS radical scavenging activities) than OP due to their lower molecular weights. POSs also increased the number of probiotics (Lactobacillus rhamnosus ATCC 7469 and Bifidobacterium longum ATCC 15707) and promoted generation of SCFAs. Furthermore, POSs with lower molecular weights inhibited LPS-induced nitrite and inflammatory cytokines (including interleukin (IL)-1β and IL-6) as well as inducible nitric oxide synthase (iNOS)/nuclear factor (NF)-κB signaling axis in RAW 264.7 cells. Therefore, it can be concluded that Fenton reaction can successfully prepare POSs having better biological activities than OP. Among three POSs produced, POS7 had the highest antioxidant and anti-inflammatory activities and good prebiotic capacity.
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•Fenton reaction (Fe2+-H2O2) successfully produced POSs from OP.•The composition and molecular weight of POSs affected their biological activities.•POSs showed good antioxidant, prebiotic, and anti-inflammatory activities.•POS with the lowest Mw and enriched RG-I showed the highest biological activities.