Prevalence and distribution pattern of Sarcocystis spp. in slaughtered cattle from the Peruvian tropical Andes, Peru

• Published in: Veterinary parasitology (Amsterdam) Vol. 48; p. 100990
• Main Authors: Jauregui, Zoilita, Salas-Fajardo, Martha Ynés, Puicón, Víctor, Lucas, J. Raul
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2024
• Subjects: Animals; Cattle; Heart - parasitology; Histopathology; Humans; Microscopy; Peru - epidemiology; Peruvian Andes; Prevalence; Sarcocystis; Sarcocystis spp; Sarcocystosis - epidemiology; Sarcocystosis - parasitology; Sarcocystosis - veterinary; Peru; Sarcocystis spp; Peruvian Andes; Histopathology; Prevalence; Microscopy; Cattle
• ISSN: 2405-9390; 2405-9390
• DOI: 10.1016/j.vprsr.2024.100990

This study aimed to estimate the prevalence and distribution patterns of Sarcocystis spp. in cattle tissues in Chachapoyas province in the Peruvian tropical Andes. Additionally, the risk factors associated with the prevalence and the correlation of two diagnostic techniques (direct microscopy of squashed fresh muscle tissues and histopathology) were explored. The tongue, heart, esophagus, Latissimus dorsi muscle, and diaphragm of 210 animals slaughtered in the municipal slaughterhouse of Chachapoyas were evaluated by both techniques. Macroscopic sarcocysts were detected in 16.7% of tissues (CI 95% 11.7–21.7%). The total prevalence of Sarcocystis spp. was 96.2% (95% CI 93.6–98.8%) by direct light microscopy and 100% by histopathology. The highest Sarcocystis prevalence was detected in the esophagus. No significant statistical differences were found in the prevalence of Sarcocystis related to sex, age, or provenance. Both techniques demonstrated a very weak Kappa correlation (κ ≤ 0.24) in predicting the presence of the parasite in each of the five evaluated muscles. Direct microscopy can be implemented at slaughterhouses as a rapid screening test, but it is essential to confirm by histopathology the absence of the parasite in direct-microscopy-negative samples. It is also recommended that beef from the Peruvian Andes be thoroughly cooked for both human and animal consumption because of the zoonotic potential of some species of Sarcocystis.
•Sarcocystis is highly endemic in cattle from the tropical Peruvian Andes.•Sarcocystis screening of cattle from this area should involve the esophagus.•Undercooked beef from the Peruvian Andes poses a potential risk for this parasite.