Decrease in expression of histamine H₂ receptors by human amniotic epithelial cells during differentiation into pancreatic beta-like cells

• Published in: Inflammation research Vol. 59; no. 2; pp. 205 - 207
• Main Authors: Szukiewicz, D, Pyzlak, M, Stangret, A, Rongies, W, Maslinska, D
• Format: Journal Article
• Language: English
• Published: Basel Basel : SP Birkhäuser Verlag Basel 01-03-2010; SP Birkhäuser Verlag Basel; Springer Nature B.V
• Subjects: Allergology; Amnion - cytology; Amnion - metabolism; Amniotic epithelial cells; Biomedical and Life Sciences; Biomedicine; C-Peptide - metabolism; Cell Differentiation - physiology; Cell Shape; Cells, Cultured; Dermatology; Epithelial Cells - metabolism; Female; Histamine receptors; Humans; Immunology; Insulin-Secreting Cells - metabolism; Insulin-Secreting Cells - physiology; Neurology; Niacinamide - pharmacology; Nicotinic Agonists - pharmacology; Pancreatic differentiation; Pharmacology/Toxicology; Pregnancy; Receptors, Histamine H1 - biosynthesis; Receptors, Histamine H2 - biosynthesis; Rheumatology; Short Communication; Histamine receptors; Amniotic epithelial cells; Pancreatic differentiation
• ISSN: 1023-3830; 1420-908X
• DOI: 10.1007/s00011-009-0131-6

Objective and design Considering the role of histaminergic pathway in the differentiation of stem cells, we compared expression patterns of H₁ and H₂ receptors in the human amniotic epithelial cells (HAEC) culture at different stages of nicotinamide-induced differentiation into PBLC with the control HAEC. Material and methods HAEC isolated after term pregnancies (N = 12) were cultured in vitro. Altogether, 72 cultures were established. The culture medium in the studied group was supplemented on Day 5 with nicotinamide (10 mM). C-peptide concentration in the medium collected every 3 days for 15 days was determined immunoenzymatically as a marker of differentiation. At the same intervals the cultures were formalin-fixed and paraffinembedded for H₁ and H₂ receptors immunostaining. Quantitative immunohistochemistry was applied for evaluation of H₁ and H₂ expression. Results C-peptide was detected on Day 6 and the levels were kept gradually increased until Day 12, then stayed at almost the same level, 3.7-fold higher than initially. Expression of H₂ was unchanged until Day 9 after nicotinamide addition, then was significantly (p < 0.05) decreased and amounted (mean % value for the measurements performed on Day 12 and Day 15, ±SEM) 49.73 ± 11.03 of the reference value obtained in control HAEC. Conclusion Variable expression of H₂ during nicotinamide-induced differentiation of HAEC into PBLC may define a time-point, indicating involvement of histamine at the earlier stages.