Effective rebaudioside a separation from stevia extracts by enzymatic bioconversion
Rebaudioside A with highly purity from commercialized stevia extracts is not amenable to preparative separation, owing to the structural similarity between stevioside and rebaudioside A, necessitating a new bioconversion method for its easy and simple separation. Complex stevia extracts were separat...
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| Published in: | Journal of food processing and preservation Vol. 45; no. 3 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-03-2021
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| Online Access: | Get full text |
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| Summary: | Rebaudioside A with highly purity from commercialized stevia extracts is not amenable to preparative separation, owing to the structural similarity between stevioside and rebaudioside A, necessitating a new bioconversion method for its easy and simple separation. Complex stevia extracts were separated soluble or insoluble fractions after bioconversion with Penicillium decumbens‐derived β‐glucosidase (SPGase). Then, rebaudioside A or steviol was main component in the water soluble or insoluble. At 100 g/L commercialized stevia extracts scale, the enzymatic bioconversion gave the rebaudioside A (6.95 g/L) from soluble with highly purity and steviol (20.45 g/L) from insoluble.
Practical applications
This one step‐enzymatic bioconversion with SPGase could be used for the separation of rebaudioside A from commercialized stevia extracts. This simple method is very useful application for low‐calorie sweetener in food materials. |
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| Bibliography: | Ha‐Nul Lee and Seong‐Jin Hong contributed equally to this work. |
| ISSN: | 0145-8892 1745-4549 |
| DOI: | 10.1111/jfpp.15202 |