Simultaneous Identification and Quantification of Three Xanthones and Two Polyisoprenylated Benzophenones in Eight Indian Garcinia Species Using a Validated UHPLC-PDA Method

• Published in: Journal of AOAC International Vol. 102; no. 5; pp. 1423 - 1434
• Main Authors: Kureshi, Azazahemad A, Dholakiya, Chirag, Hussain, Tabaruk, Mirgal, Amit, Salvi, Siddhesh P, Barua, Pritam C, Talukdar, Madhumita, Beena, Chekunnath, Kar, Ashish, Zachariah, Thondiath John, Kumari, Premlata, Dhanani, Tushar, Singh, Raghuraj, Manivel, Ponnuchamy, Kumar, Satyanshu
• Format: Journal Article
• Language: English
• Published: England 01-09-2019
• Subjects: Benzophenones - analysis; Chromatography, High Pressure Liquid - methods; Garcinia - chemistry; Xanthones - analysis
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/102.5.1423

Xanthones and polyisoprenylated benzophenones (PIBs) are two important classes of plant secondary metabolites with a wide range of bioactivities.
species synthesize numerous xanthones and PIBs. As per the literature, no data claiming simultaneous identification and quantification of three xanthones, α-mangostin, β-mangostin, γ-mangostin, and two PIBs, xanthochymol, isoxanthochymol, were found.
A validated ultra-HPLC (UHPLC)-photodiode array (PDA) method for the simultaneous identification and quantification of five compounds in different extracts of eight Indian
species was developed. The compounds were separated on a Waters ACQUITY™ UPLC H-Class column using a mobile phase consisting of solvents 0.1% formic acid in water (A) and methanol (B) in gradient elution mode. The total run time was 9 min.
From fruit rinds of eight Indian
species, namely
, and
, extracts were prepared using solvents of varying polarity. These extracts were analyzed for five biologically important compounds, namely α-mangostin, β-mangostin, γ-mangostin, xanthochymol, and isoxanthochymol. The results revealed that there is a wide variation in concentration of these compounds in extracts of
species.
The developed and validated UHPLC-PDA method could be used for simultaneous identification and quantification of these five compounds for bioprospection of other
species.