Endogenous levels of cytokinins, indoleacetic acid, abscisic acid, and pigments in variegated somaclones of micropropagated banana leaves

• Published in: Journal of plant growth regulation Vol. 17; no. 2; pp. 59 - 61
• Main Authors: Zaffari, G.R. (Experimental Station of Itajai, Itajai, SC.), Peres, L.E.P, Kerbauy, G.B
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-05-1998; Springer Nature B.V
• Subjects: ABA; ADENINA; ADENINE; Agronomy. Soil science and plant productions; AIA; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; Biological and medical sciences; CAROTENOIDE; CAROTENOIDES; CAROTENOIDS; CHEMICAL COMPOSITION; CHLOROPHYLLE; CHLOROPHYLLS; CITOQUININAS; CLOROFILAS; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CYTOKININE; CYTOKININS; Economic plant physiology; FEUILLE; Fruits; Fundamental and applied biological sciences. Psychology; Growth and development; Growth regulators; High pressure; HOJAS; IAA; Immunoassay; ISOPENTENYLADENINE; ISOPENTENYLADENOSINE; LEAVES; Liquid chromatography; MICROPROPAGACION; MICROPROPAGATION; MUSA; Pigments; PLANT GROWTH SUBSTANCES; QUANTITATIVE ANALYSIS; SOMACLONAL VARIATION; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; TISSUE CULTURE; VARIACION SOMACLONAL; VARIATION SOMACLONALE; VARIEGACION; VARIEGATION; ZEATIN; ZEATIN RIBOSIDE; ZEATINA; ZEATINE; Chlorophyll; Monocotyledones; Auxin; Musaceae; Plant leaf; Micropropagation; Concentration; Cytokinin; Plant; Musa; Endogenous; Variegation; Sesquiterpenes; Indoleacetic acid; Angiospermae; Pigments; Abscisic acid; Plant growth substance; Spermatophyta; Phytohormone; Carotenoid
• ISSN: 0721-7595; 1435-8107
• DOI: 10.1007/PL00007017

The banana (Musa spp. AAA) micropropagation shows a high incidence of off-types, among whose variegated plants are very common. Endogenous levels of growth regulators and pigment content were measured in normal and variegated leaves of the micropropagated banana plants growing in a greenhouse. Growth regulators were separated by high pressure liquid chromatography and submitted to enzyme-linked immunosorbent assay for quantification. Pigment content was measured using the colorimetric method. Green leaves contained 1.9 and 10 times more cytokinins compared with green and yellow sectors of variegated leaves, respectively. The levels of indoleacetic acid in normal leaves were significantly higher than those found in green and yellow sectors of variegated leaves; however, the levels of abscisic acid were lower in normal leaves. The lower content of chlorophylls in variegated leaves coincided with decreased endogenous levels of cytokinins, which indicated that variegation in banana leaves may be associated with alterations in the metabolism of this growth regulator