Human monoclonal antibodies to influenza virus: IgG subclass and light chain distribution

• Published in: Viral immunology Vol. 2; no. 1; p. 31
• Main Authors: Frank, A L, Six, H R, Marchini, A
• Format: Journal Article
• Language: English
• Published: United States 1989
• Subjects: Adult; Antibodies, Monoclonal - classification; Antibodies, Viral - classification; B-Lymphocytes - immunology; Cell Transformation, Viral; Clone Cells; Enzyme-Linked Immunosorbent Assay; Herpesvirus 4, Human; Humans; Immunoglobulin G - classification; Immunoglobulin Light Chains - classification; Infant; Infant, Newborn; Influenza A virus - immunology; Vaccination
• ISSN: 0882-8245
• DOI: 10.1089/vim.1989.2.31

Three adults and three children were immunized with inactivated or live attenuated influenza vaccines and 98 IgG monoclonal antibodies derived from EBV immortalization of their blood lymphocytes were studied. All antibodies reacted specifically with influenza A H3N2 or H1N1 whole virus and 73 of 74 tested reacted with the purified HA glycoproteins. The majority (76%) of 77 monoclonal antibodies adequately tested by ELISA or solid phase RIA contained lambda light chains. ELISA analysis of the IgG subclass of the six persons' postimmunization anti-influenza serum activity and of monoclonal antibodies derived from them showed a similar predominance of IgG1.