TOUCH-UP gradient amplification method

We report a unique amplification technique that works efficiently and specifically over a temperature range, rather than at one specific temperature, throughout the amplification process. As bisulfite-modified DNA is one of the difficult to amplify templates, we used this technique to amplify region...

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Bibliographic Details
Published in:	Journal of biomolecular techniques Vol. 23; no. 1; pp. 1 - 3
Main Authors:	Rowther, F B, Kardooni, H, Warr, T
Format:	Journal Article
Language:	English
Published:	United States Association of Biomolecular Resource Facilities 01-04-2012
Subjects:	Cell Line, Tumor Communication CpG Islands DNA - chemistry DNA - genetics DNA - isolation & purification DNA Methylation Genetic Loci Humans Polymerase Chain Reaction - methods Promoter Regions, Genetic Sequence Analysis, DNA Sulfites - chemistry COBRA DNA methylation analysis bisulphite modified DNA methylation specific PCR
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Summary:	We report a unique amplification technique that works efficiently and specifically over a temperature range, rather than at one specific temperature, throughout the amplification process. As bisulfite-modified DNA is one of the difficult to amplify templates, we used this technique to amplify regions of promoter-associated CpG island for 11 genes using this template. This technique amplified specific products for every gene without requiring any optimization.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	1524-0215 1943-4731
DOI:	10.7171/jbt.12-2301-004