Synthesis and Studies on Photophysical Properties of Rhodamine Derivatives for Bioimaging Applications

• Published in: Bulletin of the Korean Chemical Society Vol. 40; no. 6; pp. 554 - 559
• Main Authors: Vanjare, Balasaheb D., Mahajan, Prasad G., Dige, Nilam C., Phull, Abdul Rehman, Kim, Song Ja, Lee, Ki Hwan
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley‐VCH Verlag GmbH & Co. KGaA 01-06-2019; 대한화학회
• Subjects: Cell imaging; Cytotoxicity; Photophysical properties; Rhodamine derivatives; 화학
• ISSN: 1229-5949; 0253-2964; 1229-5949
• DOI: 10.1002/bkcs.11733

Rhodamine derivatives have been persistently used as fluorescent/solvatochromic dyes in various areas. For example, they are used as a labeling agent in biotechnology and a chemosensor. Also, rhodamine derivatives are used for cell imaging experiment. Herein, we report on the synthesis of fluorescent derivatives based on rhodamine B (RHB1, RHB2, and RHB3). We confirmed the structures and purities of the synthesized probes i.e., RHB1, RHB2, and RHB3 by using analytical technique's viz. 1H NMR, 13C NMR, Mass and FT‐IR followed by exploring spectroscopic properties by using fluorescence/UV absorption spectroscopy. The effect of solvents on the fluorescence and absorption properties of the synthesized compounds has been comprehensively studied using a series of solvent. In addition, synthesized RHB1, RHB2, and RHB3 were found to be non‐toxic against the cancer cell (MDA‐MB‐231). It is clear from the result of the conventional fluorescence microscopy that probe RHB2 exhibits significant optical properties as compared with those of RHB1 and RHB3.
Exploration of rhodamine derivatives for cell imaging studies using their optical properties.