Mixed-breed twin production by contralateral direct-transfer of male embryos to beef cow recipients bred to GnRH-based, fixed-time AI

• Published in: Livestock science Vol. 118; no. 3; pp. 272 - 281
• Main Authors: Small, J.A., Ambrose, D.J., Kastelic, J.P., Rajamahendran, R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-11-2008
• Subjects: Beef cows; Calves; Fixed-time AI; Fixed-time embryo transfer; Twinning; TET; NR; HO; Calves; CL; Twinning; ER; hCG; PGF; Beef cows; GB; Fixed-time embryo transfer; GnRH; TAI; Fixed-time AI
• ISSN: 1871-1413; 1878-0490
• DOI: 10.1016/j.livsci.2008.02.005

Two experiments were conducted to develop protocols for the use of fixed-time artificial insemination and embryo transfer (TAI and TET, respectively) to increase beef cattle productivity. Suckled beef cows were given GnRH (100 µg im) on Day − 10, and PGF (25 mg im) on Day − 3, with TAI on Day 0 (66 h later), and assigned to either embryo recipient (ER) or no embryo (NR) treatments on Days 6 or 7. Semen from Gelbvieh (GB) beef sires was used for TAI; sexed-male
in vivo developed Holstein embryos (HO) were placed nonsurgically (TET) into the uterine horn contralateral to the corpus luteum. In Experiment 1, ovarian status of cows (
n = 111; 69 ± 11 d postpartum; mean ± SD on Day 0) in Groups I and II was presynchronized with a single PGF treatment on Day − 24; Groups II and III received GnRH concurrent with TAI, and ER (
n = 78) were selected from all groups on Days 6 or 7. Neither presynchronization nor GnRH affected rates of recipient selection, Day 45 pregnancy (43.2, 43.2, and 54.0% for Groups I, II and III, respectively), or calving (40.5, 37.8, and 43.2%). However, treatment with GnRH increased HO-birth rate (8.0, 14.0 and 24.0%;
P < 0.05). In Experiment 2, cows (
n = 99, 113 ± 10 d postpartum) were assigned on Day 7 to NR and ER, with and without hCG (2500 IU im), following a TAI protocol (as per Group III in Experiment 1). Treatment with hCG increased reproductive rate (1.16 vs 1.44 calves/calving;
P < 0.05), but had no significant effect on rates of Day 45 pregnancy (45.3 vs 41.3%), calving (31.2 vs 37.0%) or HO-births (20.0 vs 26.0%). In summary, GnRH-based synchronization rates were 71.2% (based on circulating progesterone concentrations that were < 1.0 ng/mL on Day 0 and ≥ 1.0 ng/mL on Day 7); TET did not affect GB-birth rate, but more calves were produced by ER than NR cows (1.43 vs 1.02 calves/calving;
P < 0.01); and weaned calf production was 53% greater for twin- than singleton-suckled cows (392 ± 25 vs 256 ± 11 kg/dam; mean ± SEM,
P < 0.05). Therefore, GnRH-based TAI and TET protocols for mixed-breed twin production increased beef cow productivity.