Fabrication of palladium nanoparticles anchored polypyrrole functionalized reduced graphene oxide nanocomposite for antibiofilm associated orthopedic tissue engineering

• Published in: Applied surface science Vol. 510; p. 145403
• Main Authors: Murugesan, Balaji, Pandiyan, Nithya, Arumugam, Mayakrishnan, Sonamuthu, Jegatheeswaran, Samayanan, Selvam, Yurong, Cai, Juming, Yao, Mahalingam, Sundrarajan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30-04-2020
• Subjects: Antibiofilm associated bone tissue engineering; Graphene; Palladium; Pd/PPy/rGO nanocomposite; Polypyrrole; Pd/PPy/rGO nanocomposite; Palladium; Graphene; Antibiofilm associated bone tissue engineering; Polypyrrole
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2020.145403

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•The Pd/PPy/rGO NC was synthesized by oxidative polymerization with PdCl2 reduction.•The XPS spectrum confirms that C1s, N1s, O1s, and Pd3d are present in the sample.•The SEM and TEM micrographs confirm 2–5 nm size Pd NPs are dispersed on PPy/rGO.•It successfully inhibits the biofilm formed by pathogenic bacteria.•It shows appropriate cytocompatibility for osteo cell proliferation and adhesion.
Two-dimensional reduced graphene oxide (rGO) was used as a scaffold for bone tissue engineering application. To advance the biological properties including biocompatibility, osteoproliferation, and prevent bacterial infection, the rGO was non-covalently functionalized with polypyrrole (PPy) and palladium (Pd) nanoparticles (NPs). The results propose that PPy and Pd in the Pd/PPy/rGO nanocomposite (NC) create the surface functionalities and binding sites to enhance the physicochemical properties of rGO for biomedical applications. X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis confirmed the highly dispersed 2–5 nm-sized Pd NPs distributed and anchored on the PPy/rGO surface. The Fourier-transform infrared (FTIR) spectrum shows a peak at 1672 cm−1 corresponds to Pd-nitrogen stretching band which is caused by the interaction between Pd and PPy matrix. Moreover, X-ray photoelectron spectroscopy (XPS) approves the zero oxidation state of Pd (Pd0) NPs. It successfully prevents bacterial biofilms caused by E. coli, B. subtilis, P. aeruginosa, and K. pneumoniae. Therefore the NC may be used to build a bone implant material that ensures prevention from colonizing, adhering, and forming microbial biofilms on the substrates of such materials. In the future, Pd/PPy/rGO NC can be a promising material for pharmaceutical applications such as drug-delivery, anticancer and wound healing agents.