Effectiveness of Psychobiotic Bifidobacterium breve BB05 in Managing Psychosomatic Diarrhea in College Students by Regulating Gut Microbiota: A Randomized, Double-Blind, Placebo-Controlled Trial

• Published in: Nutrients Vol. 16; no. 13; p. 1989
• Main Authors: Wang, Yufan, Wang, Yufei, Ding, Kunpeng, Liu, Yuhan, Liu, Dingming, Chen, Weijun, Zhang, Xinyi, Luo, Chuanlin, Zhang, Hongyan, Xu, Tangchang, Chen, Tingtao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22-06-2024
• Subjects: Adult; Anxiety; anxiety and depression; Bifidobacterium breve; bifidobacterium breve bb05; College students; dcs; Diarrhea; Diarrhea - microbiology; Diarrhea - therapy; Double-Blind Method; Experiments; Feces; Feces - microbiology; Female; Gastrointestinal Microbiome; gut microbiota; Humans; Male; Mental depression; Mental disorders; Metabolites; Microbiota; microbiota–gut–brain axis; Pathogens; Probiotics; Probiotics - administration & dosage; Probiotics - therapeutic use; Questionnaires; Students - psychology; Universities; Young Adult; China; bifidobacterium breve bb05; microbiota–gut–brain axis; anxiety and depression; dcs; gut microbiota
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16131989

Diarrhea of college students (DCS) is a prevalent issue among college students, affecting their daily lives and academic performance. This study aims to explore the potential effect of
BB05 supplements on the DCS. Initially, fifty healthy and fifty diarrheal students were recruited in the observational experiment and allocated into control and diarrhea groups, respectively. Subsequently, one hundred diarrheal students were newly recruited in the intervention experiment and randomly allocated into placebo and probiotic groups, both treated for 2 weeks. Questionnaires (BSS, HAMA-14, and HDRS-17) were performed to assess the students' diarrheal states and mental health at baseline and post-treatment. Fecal samples underwent 16S rRNA sequencing and Enzyme-Linked Immunosorbent Assay to evaluate gut microbiota and fecal metabolite alternations. Results indicated that
BB05 supplementation significantly enriched (
< 0.05) the reduced gut microbial diversity caused by diarrhea. Diarrhea resulted in notable alterations in gut microbiota composition, as exhibited by elevated
and
, alongside substantially decreased
,
, and
, while
BB05 supplementation partially restored the compromised gut microbiota at both the phylum and genus levels, particularly by increasing
and
(
< 0.05). Importantly, questionnaire results suggested that
BB05 administration achieved superior efficacy in relieving diarrhea symptoms and the associated anxiety and depression in college students. An increased fecal concentration of 5-hydroxytryptamine (5-HT) was also observed in the probiotic group, while Acetylcholine (ACH), Epinephrine (EPI), and Noradrenaline/Norepinephrine (NANE) reduced, revealing the potential of
BB05 in alleviating anxiety and depression via modulating the microbiota-gut-brain axis. Furthermore, correlation analysis suggested that the altered microbiota and fecal neurotransmitters were closely associated with the mental symptoms. These results endorse
BB05 intervention as a promising and innovative approach to alleviate both diarrhea and mental health conditions among college students.