Evaluation of expression level of BANCR, MALAT1 and FER1L4 and their target genes in coumarin-treated AGS cell line

• Published in: Pathology, research and practice Vol. 257; p. 155291
• Main Authors: Shafaee Arani, Shirin, Nejati, Majid, Rastgoufar, Sepide, Raisi, Arash, Eshraghi, Reza, Ostadian, Amirreza, Matini, Amir Hassan, Rahimain, Neda, Mirzaei, Hamed
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-05-2024
• Subjects: BANCR; Coumarin; FER1L4; Gastric cancer; Long non-coding RNAs; MALAT1; Long non-coding RNAs; MALAT1; FER1L4; Gastric cancer; Coumarin; BANCR
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/j.prp.2024.155291

Because long non-coding RNAs (lncRNAs) can affect several interconnected processes, its value as a predictive marker for gastric cancer has been demonstrated. Coumarin - a natural compound known to contain some beneficial antitumor qualities - was tested for its effects on AGS gastric cancer cells. In this study, we investigated the expression level of selected cellular lncRNAs (BANCR, MALAT1 and FER1L4) and their target genes (PTEN, p-PI3K and p-AKT) in coumarin-treated AGS cell line. The expressions of the three lncRNAs: BANCR, MALAT1 and FER1L4, as well as their specified targets, PTEN, PI3K and AKT, were measured by qRT-PCR. To gauge the impact of coumarin on the AGS cells, a MTT assay was utilized. A Western blot has been employed to assess variations in PTEN, p-PI3K, and p-AKT expression. The experiment’s results showed that AGS viability diminished with increasing doses of coumarin. Compared to the control cells, the cells exposed to coumarin had showed reduced levels of mRNAs which are known targets of the lncRNA BANCR. At the same time, levels of lncRNAs MALAT1 and FER1L4 within coumarin group have been higher comparing to those within control group. Additionally, the Western blot analysis revealed that the coumarin-treated cells expressed lower levels of p-PI3K, PTEN as well as p-AKT compared to control group. This information points to coumarin being a possible option in a treatment regimen for gastric cancer due to its ability to affect lncRNAs and the molecules they target.