Glucose-6-phosphate dehydrogenase promotes the infection of Chilli veinal mottle virus through affecting ROS signaling in Nicotiana benthamiana

• Published in: Planta Vol. 256; no. 5; p. 96
• Main Authors: Yang, Daoyong, Peng, Qiding, Cheng, Yongchao, Xi, Dehui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2022; Springer Nature B.V
• Subjects: Accumulation; Agriculture; Biomedical and Life Sciences; Catalase; Catalase - metabolism; Dehydrogenase; Dehydrogenases; Ecology; Ethylenes - metabolism; Forestry; Gene expression; Gene Expression Regulation, Plant; Glucose 6 phosphate dehydrogenase; Glucosephosphate dehydrogenase; Glucosephosphate Dehydrogenase - genetics; Glucosephosphate Dehydrogenase - metabolism; Glutathione; Glutathione - metabolism; Homeostasis; Homology; Infections; Jasmonic acid; Life Sciences; Localization; Nicotiana - metabolism; Nicotiana benthamiana; Original Article; Oxidative metabolism; Pentose; Pentose phosphate pathway; Physiology; Plant Diseases; Plant growth; Plant Growth Regulators - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Potyvirus; Proline - metabolism; Protoplasm; Reactive oxygen species; Reactive Oxygen Species - metabolism; Respiratory burst oxidase; Signal Transduction; Signaling; Viruses; NbG6PDH; Reactive oxygen species; Jasmonic acid/ethylene signaling; ChiVMV; Anti-oxidative metabolism
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-022-04010-1

Main conclusion
G6PDH negatively regulates viral accumulation in
Nicotiana benthamiana
through RBOHB-associated ROS signaling. Anti-oxidative metabolism and phytohormone-mediated immunity responses play important roles in virus infection.
Glucose-6-phosphate dehydrogenase (G6PDH) is an enzyme in the pentose phosphate pathway, which plays an important role in maintaining intracellular redox homeostasis and has functions in plant growth, development and stress tolerance. However, the role of G6PDH in plants response to virus infection is poorly understood. In this study, NbG6PDH was found to be down-regulated after Chilli veinal mottle virus (ChiVMV-GFP) infection in
Nicotiana benthamiana
. Subcellular localization of NbG6PDH showed that it was punctate distributed in the protoplasm. Silencing of NbG6PDH reduced the sensitivity of
N. benthamiana
plants to ChiVMV-GFP. By contrast, transient overexpression of NbG6PDH promoted the accumulation of the virus. The results of physiological indexes showed that glutathione (GSH), catalase (CAT) and proline played an important role in maintaining plants physiological homeostasis. The results of gene expression detection showed that jasmonic acid/ethylene (JA/ET) signaling pathway was significantly correlated with the response of
N. benthamiana
to ChiVMV-GFP infection, and the changes of
N. benthamiana
respiratory burst oxidase homologues B (NbRBOHB) indicated that the NbG6PDH-dependent ROS may be regulated by NbRBOHB. Pretreatment of the inducer of reactive oxygen species (ROS) promoted virus infection, whereas inhibitor of ROS alleviated virus infection. Thus, our results indicate that the promoting effect of NbG6PDH on ChiVMV-GFP infection may be related to the NbRBOHB-regulated ROS production.