In vitro modulation of the expression of 15-hydroxy-prostaglandin dehydrogenase by trophoblast differentiation

• Published in: American journal of obstetrics and gynecology Vol. 180; no. 3; pp. 690 - 695
• Main Authors: Lennon, Carol, Carlsona, Matthew G., Nelson, D.Michael, Sadovsky, Yoel
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Philadelphia, PA Elsevier Inc 01-03-1999; Elsevier
• Subjects: 15-Hydroxy-prostaglandin dehydrogenase; 8-Bromo Cyclic Adenosine Monophosphate - pharmacology; Biological and medical sciences; Blotting, Western; Cell Differentiation; Cells, Cultured; Culture Media; cyclic adenosine monophosphate; Cyclic AMP - physiology; Female; Fundamental and applied biological sciences. Psychology; Humans; Hydroxyprostaglandin Dehydrogenases - biosynthesis; Immunoenzyme Techniques; Mother. Fetoplacental unit. Mammary gland. Milk; placenta; Pregnancy; Pregnancy. Parturition. Lactation; prostaglandin; trophoblast; Trophoblasts - cytology; Trophoblasts - enzymology; Vertebrates: reproduction; placenta; cyclic adenosine monophosphate; 15-Hydroxy-prostaglandin dehydrogenase; trophoblast; prostaglandin; Human; Trophoblaste; Placenta; Enzyme; Female; Oxidoreductases; 3α-Hydroxysteroid dehydrogenase (B-specific); Cell differentiation; In vitro; Biological activity
• ISSN: 0002-9378; 1097-6868
• DOI: 10.1016/S0002-9378(99)70274-7

Objective: Our goal was to determine the expression and activity of 15-hydroxy-prostaglandin dehydrogenase, a prostaglandin-metabolizing enzyme, in differentiating trophoblasts in vitro. Study Design: Cytotrophoblasts from placentas of term healthy women were cultured in either Ham’s-Waymouth medium, which hinders the process of cytotrophoblast differentiation, or medium 199, which facilitates differentiation into syncytiotrophoblasts. 15-Hydroxy-prostaglandin dehydrogenase expression was determined with Western immunoblotting, and activity was measured by a specific enzyme immunoassay of 13,14-dihydro-15-keto prostaglandin F2α , an inactive product of 15-hydroxy-prostaglandin dehydrogenase activity. Results: The expression and activity of 15-hydroxy-prostaglandin dehydrogenase were enhanced during trophoblast differentiation and were higher in cells grown in medium 199 than in those grown in Ham’s-Waymouth medium. 8-Bromo-cyclic adenosine monophosphate, which stimulates prostaglandin H synthase-2 expression, diminished the expression and activity of 15-hydroxy-prostaglandin dehydrogenase in concentration- and time-dependent manners. Conclusions: 15-Hydroxy-prostaglandin dehydrogenase expression and activity are regulated during trophoblast differentiation and by cyclic adenosine monophosphate. Coordinated expression of l5-hydroxy-prostaglandin dehydrogenase and prostaglandin H synthase-2 contributes to the regulation of prostaglandin release from trophoblasts. (Am J Obstet Gynecol 1999;180:690-5.)