Green and inventive fluorescence approach for levodropropizine determination in human plasma
[Display omitted] •The first green fluorescence approach was presented for the detection of levodropropizine.•The drug's assay was conducted in spiked human plasma.•Samples were utilized without undergoing any pre-treatment or extraction procedures.•This method presents a new option for perform...
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| Published in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 312; p. 124060 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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Elsevier B.V
05-05-2024
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| Abstract | [Display omitted]
•The first green fluorescence approach was presented for the detection of levodropropizine.•The drug's assay was conducted in spiked human plasma.•Samples were utilized without undergoing any pre-treatment or extraction procedures.•This method presents a new option for performing bioavailability investigations of levodropropizine.
A green, rapid and sensitive fluorimetric method to quantify levodropropizine (LVP) in human plasma was exploited for the first time. The proposed method adopts LVP's intrinsic fluorescence in distilled water at a detecting emission of 345 nm following excitation at 240 nm. LVP displayed linearity across concentrations ranging from 50 to 1000 ng mL−1, with a detection limit of 0.77 ng mL−1 and a quantification limit of 2.33 ng mL−1. Thorough validation confirmed its reliability, successfully determining LVP in tablets with an average recovery of 98.64 ± 1.07 %. Furthermore, the method's applicability extended to estimate the studied drug in spiked human plasma with excellent obtained percentage recoveries (98.68 ± 1.28–100.14 ± 1.23). |
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| AbstractList | [Display omitted]
•The first green fluorescence approach was presented for the detection of levodropropizine.•The drug's assay was conducted in spiked human plasma.•Samples were utilized without undergoing any pre-treatment or extraction procedures.•This method presents a new option for performing bioavailability investigations of levodropropizine.
A green, rapid and sensitive fluorimetric method to quantify levodropropizine (LVP) in human plasma was exploited for the first time. The proposed method adopts LVP's intrinsic fluorescence in distilled water at a detecting emission of 345 nm following excitation at 240 nm. LVP displayed linearity across concentrations ranging from 50 to 1000 ng mL−1, with a detection limit of 0.77 ng mL−1 and a quantification limit of 2.33 ng mL−1. Thorough validation confirmed its reliability, successfully determining LVP in tablets with an average recovery of 98.64 ± 1.07 %. Furthermore, the method's applicability extended to estimate the studied drug in spiked human plasma with excellent obtained percentage recoveries (98.68 ± 1.28–100.14 ± 1.23). |
| ArticleNumber | 124060 |
| Author | Mostafa, Islam M. Ahmed Elsayed, Mohamed Mohamed, Abobakr A. Omar, Mahmoud A. Almaghrabi, Mohammed |
| Author_xml | – sequence: 1 givenname: Islam M. surname: Mostafa fullname: Mostafa, Islam M. email: dr_islam@mail.ustc.edu.cn organization: Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61511, Egypt – sequence: 2 givenname: Mahmoud A. surname: Omar fullname: Omar, Mahmoud A. organization: Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al-Madinah Al-Munawwarah 41477, Saudi Arabia – sequence: 3 givenname: Mohamed surname: Ahmed Elsayed fullname: Ahmed Elsayed, Mohamed organization: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, 63514, Egypt – sequence: 4 givenname: Mohammed surname: Almaghrabi fullname: Almaghrabi, Mohammed organization: Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al-Madinah Al-Munawwarah 41477, Saudi Arabia – sequence: 5 givenname: Abobakr A. surname: Mohamed fullname: Mohamed, Abobakr A. organization: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, 63514, Egypt |
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| Keywords | Fluorimetric Levodropropizine Intrinsic fluorescence Spiked Human Plasma Tablets |
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•The first green fluorescence approach was presented for the detection of levodropropizine.•The drug's assay was conducted in spiked human... |
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| Title | Green and inventive fluorescence approach for levodropropizine determination in human plasma |
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