Green and inventive fluorescence approach for levodropropizine determination in human plasma

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 312; p. 124060
• Main Authors: Mostafa, Islam M., Omar, Mahmoud A., Ahmed Elsayed, Mohamed, Almaghrabi, Mohammed, Mohamed, Abobakr A.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05-05-2024
• Subjects: Fluorimetric; Intrinsic fluorescence; Levodropropizine; Spiked Human Plasma; Tablets; Fluorimetric; Levodropropizine; Intrinsic fluorescence; Spiked Human Plasma; Tablets
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124060

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•The first green fluorescence approach was presented for the detection of levodropropizine.•The drug's assay was conducted in spiked human plasma.•Samples were utilized without undergoing any pre-treatment or extraction procedures.•This method presents a new option for performing bioavailability investigations of levodropropizine.
A green, rapid and sensitive fluorimetric method to quantify levodropropizine (LVP) in human plasma was exploited for the first time. The proposed method adopts LVP's intrinsic fluorescence in distilled water at a detecting emission of 345 nm following excitation at 240 nm. LVP displayed linearity across concentrations ranging from 50 to 1000 ng mL−1, with a detection limit of 0.77 ng mL−1 and a quantification limit of 2.33 ng mL−1. Thorough validation confirmed its reliability, successfully determining LVP in tablets with an average recovery of 98.64 ± 1.07 %. Furthermore, the method's applicability extended to estimate the studied drug in spiked human plasma with excellent obtained percentage recoveries (98.68 ± 1.28–100.14 ± 1.23).