Improvement of Bioactive Compounds by Exogenous Sucrose Absorbed via Mugwort and Kale Roots

• Published in: Weon'ye gwahag gi'sulji Vol. 37; no. 1; pp. 1 - 9
• Main Authors: Yeom, Moon-Sun, Jeong, Hye-Jin, Lee, Jun-Soo, Oh, Myung-Min
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 01-02-2019; 한국원예학회
• Subjects: 농학; total phenolic content; ethanol-induced cytotoxicity; sugar analysis; Artemisia princeps; Brassica oleracea var. acephala; antioxidant capacity
• ISSN: 1226-8763; 2465-8588
• DOI: 10.12972/kjhst.20190001

In plants, sucrose (suc) acts as a signaling molecule for various plant metabolism processes and isone of the translocation forms of carbohydrates. This study aimed to determine the effect of exogenoussuc on the accumulation of antioxidative phenolics in mugwort (Artemisia princeps) and kale(Brassica oleracea var. acephala) plants and to evaluate the protective effect of mugwort extracttreated with suc on ethanol-induced cell damage. Two-week-cultured mugwort plants and threeweek-cultured kale plants were subjected to Hoagland nutrient solution containing 10, 30, and 50 mMsuc for 4 and 3 days. As a result, the shoot fresh weights in the suc treatments were not significantlydifferent from the control in both plants. However, the sugar content in kale leaves in all treatmentswas significantly higher than the control at 3 days of treatment. The total phenolic content andantioxidant capacity of mugwort increased as suc concentration increased. For kale, the suc 30 mMtreatment had significantly higher antioxidant capacity than the control at 1 and 3 days of treatment.
However, the other suc concentrations did not affect the change of antioxidant capacity. Totalphenolic content in the kale plants was not significantly affected by the exogenous suc addition. Theprotective effects of the extracts from the suc-treated mugwort were examined by MTT assayagainst ethanol-induced cell damage. Ethanol-induced cell damage was observed in the ethanoltreatment; however, pretreatment of the extract significantly reduced the ethanol-induced cytotoxicity.
Therefore, the kale and mugwort plants could absorb exogenous suc via roots, and suc mightstimulate the biosynthesis of antioxidant phenolic compounds or certain bioactive compounds,efficiently protecting hepatocytes against ethanol-induced cell damage in mugwort. KCI Citation Count: 0