A PNA-DNA linker synthesis of N-((4,4′-dimethoxytrityloxy)ethyl)- N-(thymin-1-ylacetyl)glycine

• Published in: Bioorganic & medicinal chemistry letters Vol. 5; no. 11; pp. 1119 - 1124
• Main Authors: Petersen, Kenneth H., Jensen, Dorte K., Egholm, Michael, Nielsen, Peter E., Buchardt, Ole
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 08-06-1995
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/0960-894X(95)00177-U

N-(2-benzyloxyethyl)-
N-(thymin-1-ylacetyl)glycine was synthesised and used as a linker for coupling DNA and PNA together. Three different 10-mers composed of 6 DNA units and 4 PNA thymine units were made. The PNA-DNA chimera hybridise to complementary DNA or PNA oligomers, and both the PNA and the DNA part of the chimera are involved in the binding.
N-(2-benzyloxyethyl)-
N-(thymin-1-ylacetyl)glycine was synthesised and used as a linker for coupling DNA and PNA together. Three different 10-mers were made. They all hybridise to the complementary DNA or PNA and both parts of the chimera are involved in the binding.