Population Dynamics, Effective Soil Factors, and LAMP Detection Systems for Phytophthora Species Associated with Kiwifruit Diseases in China

• Published in: Plant disease Vol. 106; no. 3; p. 846
• Main Authors: Li, Mingzhu, Guo, Qian, Liang, Mengyi, Zhao, Qing, Lin, Tao, Gao, Han, Hieno, Ayaka, Kageyama, Koji, Zhang, Xin, Cui, Langjun, Yan, Yaping, Qiang, Yi
• Format: Journal Article
• Language: English
• Published: United States 01-03-2022
• Subjects: Molecular Diagnostic Techniques; Multiplex Polymerase Chain Reaction; Nucleic Acid Amplification Techniques; Phytophthora - genetics; Population Dynamics; Soil; redundancy analysis; LAMP detection; soil factors; tigA; qPCR; Phytophthora; Ypt1; population dynamics
• ISSN: 0191-2917
• DOI: 10.1094/PDIS-04-21-0852-RE

China has the largest area of kiwifruit production in the world. Pathogens associated with root diseases of kiwi trees have not been investigated extensively. In this research, three
species,
,
, and
, which are pathogenic to kiwi trees in the main planting areas of China, were studied. The population densities of these species in 128 soil samples from 32 kiwi orchards in 2017 and 2018 were measured using multiplex real-time quantitative PCR based on the ras-related protein gene
.
was the most widely distributed of the three species in orchards of the Zhouzhi and Meixian prefectures. We used redundancy analysis to examine soil factors in the kiwi orchards to understand their effects on the population densities of the
species. The redundancy analysis indicated that soil temperature and pH were significantly correlated with the abundance of
and
. In addition, two loop-mediated isothermal amplification detection systems for
were developed based on the
gene. The color-change detection system proved to be accurate, sensitive, and faster than quantitative PCR. The results of this study, along with the loop-mediated isothermal amplification detection systems, will be of great use in the control of Phytophthora diseases for the production of kiwifruits in China.