Using oil immersion to deliver a naturally-derived, stable bromoform product from the red seaweed Asparagopsis taxiformis

• Published in: Algal research (Amsterdam) Vol. 51; p. 102065
• Main Authors: Magnusson, Marie, Vucko, Matthew J., Neoh, Tze Loon, de Nys, Rocky
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-10-2020
• Subjects: Agriculture; Anti-methanogenic; Greenhouse gas emissions; Methanogenesis; Ruminant; Agriculture; Greenhouse gas emissions; Ruminant; Anti-methanogenic; Methanogenesis
• ISSN: 2211-9264; 2211-9264
• DOI: 10.1016/j.algal.2020.102065

Asparagopsis taxiformis (Asparagopsis) inhibits the production of enteric methane in ruminants. A next critical step in the implementation of this technology is the delivery of a naturally-derived product that maximises the concentration and longer-term retention of bromoform. This study (1) quantified the effects of solvent (water or oil), initial processing (intact or homogenised), and temperature (4 or 25 °C) on the stabilisation of bromoform over time, and (2) assessed the effects of increasing the biomass loading (g biomass mL−1 solvent) of Asparagopsis on the concentration of bromoform in a formulation. The most effective method was to homogenise freshly-collected Asparagopsis in oil, which resulted in the highest concentration of bromoform (19.2 ± 2.1 mg g−1 dw algae) in the homogeneous product in the shortest time (one day). In addition, the final product had a shelf life of at least 12 weeks, even when stored at room temperature (25 °C). Notably, there was an increase in the concentration of bromoform per mL of oil between each increment of biomass loading tested, with the highest concentration of bromoform of 4.04 ± 0.51 mg mL−1 in the maximum ratio of biomass to oil of 120 g 100 mL−1. The method described here provides a viable processing alternative to freeze-drying, resulting in the stabilisation of the bromoform from Asparagopsis, which will be critical to the success of using Asparagopsis on a larger scale to mitigate the production of methane in ruminants.
•We developed a novel method to stabilise bromoform (CHBr3) in algal biomass.•Homogenisation of Asparagopsis seaweed in vegetable oil resulted in highest CHBr3.•Shelf life of oil product stored at 25 °C was at least 12 weeks with no CHBr3 lost.•Conversely, losses of CHBr3 from freezedried Asparagopsis stored at 25 °C were 37.8%.•The novel method can facilitate use of Asparagopsis for ruminant methane mitigation.