Antimicrobial mangosteen extract infused alginate-coated gauze wound dressing

• Published in: Journal of drug delivery science and technology Vol. 41; pp. 182 - 190
• Main Authors: Panawes, Saikhim, Ekabutr, Pongpol, Niamlang, Pimolpun, Pavasant, Prasit, Chuysinuan, Piyachat, Supaphol, Pitt
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-10-2017
• Subjects: Alginate; Antibacterial activity; Gauze; Mangosteen extract; Wound dressing; Mangosteen extract; Gauze; Antibacterial activity; Alginate; Wound dressing
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2017.06.021

In the present study an antimicrobial wound dressing incorporating the fruit hull of Garcinia mangostana (MT) extracts into sodium alginate (AG)-coated gauze was developed. The effects of varying concentrations of AG on AG-coated gauze samples were studied in terms of swelling ratio, moist environment and blood coagulation properties. Furthermore, measurement of drug release from the AG-MT gauze, antibacterial activity, and in vitro biocompatibility in keratinocyte (HaCaT) cells and human dermal fibroblasts (HDFa) was assessed. With increasing AG concentration, the AG-gauze showed a higher swelling ratio, briefer moist environment as well as lower blood coagulation results. Compared with other formulations, AG-gauze with 0.5% w/w of AG was selected for incorporation with MT extract. The combination of MT extract was shown to improve antimicrobial efficacy in comparison with the control. The cumulative release of MT extract was found to be 50–55% of the total load after 90 h in an acetate buffer solution and 80–95% in a phosphate buffer solution. An in vitro cytotoxic test showed that 0.01 and 0.02% w/w MT extract loaded AG-gauze were not toxic to HaCaT and HDFa cells. Thus, AG-coated gauze containing MT extract could be a promising candidate for use in wound care.
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