Resveratrol-loaded PLGA nanoparticles functionalized with red blood cell membranes as a biomimetic delivery system for prolonged circulation time

• Published in: Journal of drug delivery science and technology Vol. 54; p. 101369
• Main Authors: Li, Chunhong, Wang, Xia, Li, Rongjun, Yang, Xiaoli, Zhong, Zhirong, Dai, Yan, Fan, Qingze, Lin, Yan, Zhang, Rongtao, Liang, Tiantian, Ye, Yun, Zhou, Meiling
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2019
• Subjects: Biomimetic; PLGA nanoparticles; Prolonged circulation; Red blood cell membranes; Resveratrol; Red blood cell membranes; Biomimetic; Prolonged circulation; PLGA nanoparticles; Resveratrol
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2019.101369

Resveratrol (RES) shows excellent pharmacological activities but is limited by a very short half-life. In this study, red blood cell (RBC) membranes camouflaging RES-loaded PLGA NPs (RBC-RES-NPs) were successfully designed and synthesized. The resulting RBC-RES-NPs were spherical and had a clear core-shell structure with an average size of 148.1 ± 1.1 nm and a negatively charged surface of −18.20 ± 0.73 mV. The entrapment efficiency and drug loading rate were 96.51 ± 1.29% and 1.79 ± 0.02%, respectively. The diameter and distribution of the RBC-RES-NPs were largely stable for 7 consecutive days. Compared with that of RES and the RES-NPs, a remarkably sustained release effect was observed in the RBC-RES-NP group, with a cumulative release rate of approximately 60% after 2 h of measurement time. After systemic injection in rats, the half-life of the RBC-RES-NPs (10.34 ± 1.40 min) was distinctly longer than that of RES (7.50 ± 1.00 min, p < 0.01) and the RES-NPs (7.31 ± 1.37 min, p < 0.01). Additionally, the mean residence time of the RBC-RES-NPs was significantly extended. These results suggest that functionalization with RBC membranes as a biomimetic delivery system represents an efficient strategy to prolong the circulation time of RES, which may lead to an increase in the therapeutic effect of RES.
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