Investigating the relationship between the VNTR variant of the interleukin-1 receptor antagonist gene and coronary in-stent restenosis

• Published in: Molecular biology reports Vol. 50; no. 10; pp. 8575 - 8587
• Main Authors: Klashami, Zeynab Nickhah, Mostafavi, Atoosa, Roudbordeh, Majid Gholamzadeh, Abbasi, Ali, Ebrahimi, Pirooz, Asadi, Mojgan, Amoli, Mahsa M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2023; Springer Nature B.V
• Subjects: Alleles; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Cytokines; Histology; IL-1β; Implants; Inflammatory diseases; Interleukin 1; Interleukin 1 receptor antagonist; Interleukin 1 receptors; Life Sciences; Morphology; Original Article; Polymerase chain reaction; Restenosis; Statistical analysis; Variable number of tandem repeats; Coronary artery disease; Interleukin-1 receptor antagonist; Atherosclerosis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-023-08759-w

Objective
This study aimed to examine the association between the interleukin-1 receptor antagonist gene (IL-1RN) and coronary in-stent restenosis (ISR) through the analysis of the VNTR variant based on the previously reported results.
Materials and methods
The samples were classified into two clearly defined groups: the case group, which comprised 45 patients diagnosed with in-stent restenosis (ISR+), and the control group, which included 60 patients without ISR (ISR-). Polymerase chain reaction (PCR) was performed to examine the 86-bp VNTR variant of the IL-1RN gene.
Results
In the analysis of six identified groups consisting of variant alleles of 86 base pairs of VNTR of the IL-1RN gene statistically significant difference was observed for the presence of IL1RN*2 allele between cases and controls (p = 0.04, OR; 0.045).
Conclusion
Individuals with allele 2 of the IL-1Ra gene may be more predisposed to ISR. This could be due to an imbalance between IL-1Ra and IL-1β which is crucial in preventing the initiation or advancement of inflammatory diseases in specific organs. The observed phenomenon can be characterized by increased production of IL-1β and potential reduction of IL-1Ra as a result of functional VNTR variation in IL-RN gene.