Inhibition of α‐Glycosidase by Lippia dulcis Trevir. (Verbenaceae) Preparations, Quantification of Verbascoside, and Study of Its Molecular Docking

• Published in: Chemistry & biodiversity Vol. 20; no. 2; pp. e202200760 - n/a
• Main Authors: Ritter Ruas, Natalia, Carvalho Pereira, Aline, Lopes Silva Pereira, Luciana, Mesquita Germano, Carolina, Fontes Ferreira da Cunha, Elaine, Alves de Carvalho, Alexandre, Alves Lameira, Osmar, Eduardo Brasil Pereira Pinto, José, Kelly Vilela Bertolucci, Suzan
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01-02-2023
• Subjects: alpha-Glucosidases - chemistry; capim doce; Diabetes mellitus; Ethanol; Glucosidase; glucosidase inhibitors; Glutathione; Glycosidases; Glycoside Hydrolase Inhibitors - chemistry; Glycoside Hydrolases; Hyaluronoglucosaminidase; Hypoglycemia; hypoglycemic agent; Hypoglycemic Agents - chemistry; Lippia; medicinal plant; Molecular docking; Molecular Docking Simulation; phenylethanoid glycoside; Plant Extracts - chemistry; Substrates; Verbenaceae; medicinal plant; phenylethanoid glycoside; glucosidase inhibitors; hypoglycemic agent; capim doce
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.202200760

This study aimed to quantify verbascoside (VEB), perform molecular docking studies of VEB with the α‐glucosidase (GL) of Bacillus stearothermophilus, and evaluate the inhibition of the enzyme by L. dulcis preparations. The substrate concentration and presence of reduced glutathione were evaluated for their effect on the in vitro inhibition of the GL enzyme. Assays were also performed in the presence and absence of simulated gastric fluid. The antidiabetic fractions 2 and 3 were the most inhibited GL, but their activity were significantly decreased in the presence of gastric fluid. Chromatographic analyses confirmed the predominant presence of VEB in the samples. The samples had VEB concentrations between 49.9 and 243.5 mg/g. Simulation of the molecular docking of VEB were consistent with its GL‐inhibitory activity. It can conclude that the crude ethanol extract and fractions show inhibitory activity against the GL enzyme.