The fate of hepatocyte cell line derived from a liver injury model with long-term in vitro passage

• Published in: Molecular & cellular toxicology Vol. 14; no. 3; pp. 263 - 272
• Main Authors: Chand, Lokendra, Risal, Prabodh, Shrestha, Nirajan, Acharya, Dhruba, Jeong, Yae Sul, Kim, Chan Young, Jeong, Yeon Jun
• Format: Journal Article
• Language: English
• Published: Incheon The Korean Society of Toxicogenomics and Toxicoproteomics 01-07-2018; Springer Nature B.V; 대한독성 유전단백체 학회
• Subjects: Biomedical and Life Sciences; Cell Biology; Cell culture; Collagen; Collagenase; Hepatocytes; Life Sciences; Liver; Liver diseases; Liver transplantation; Morphology; Organ donors; Original Paper; Perfusion; Pharmacology/Toxicology; Physical characteristics; 생물학; 3,5-diethoxycarbonyl-1,4-dihydro-collidine; Orthotopic liver transplantation; Hepatocyte transplantation
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-018-0029-x

Backgrounds
Orthotopic Liver Transplantation (OLT) is the therapy of choice for the treatment of end-stage liver disease, but the severe shortage of organ donors, complex and expensive surgical procedure and increased mortality of prospective organ recipient limit the use of OLT. To overcome this problem the technique of hepatocyte transplantation has been considered as an alternative to OLT. Hepatocyte transplantation is less invasive, cost effective cryo-preservable and can be distributed from single donor to multiple recipients. In this study we have established and characterize the hepatocyte cell line possessing the morphological and functional characteristics of hepatocytes from chemically injured liver. Hence we hypothesized that the hepatocyte cell line derived from liver injury can be used for hepatocyte transplantation.
Methods
To induce the priming of hepatocyte, mice were fed with 3,5-diethoxycarbonyl-1,4-dihydro-collidine (DDC) diet for 3 weeks and hepatocytes were obtained by two step collagenase perfusion method, hepatocytes hence obtained was expended by >300 passages and tested for various hepatocyte specific functions.
Results
The cell line derived from liver injury model retains morphological and functional characteristics of hepatocytes in long-term
in vitro
culture. These cells transplanted to mice showed significant survival rate.
Conclusion
The Hepatocyte cell line derived from liver injury model were used for hepatocyte transplantation and showed the significantly higher survival rate.