Troglitazone, a Selective Ligand for PPARγ, Induces Cell-cycle Arrest in Human Oral SCC Cells

• Published in: Anticancer research Vol. 40; no. 3; pp. 1247 - 1254
• Main Authors: Saito, Masahiro, Fujita, Yuki, Kuribayashi, Nobuyuki, Uchida, Daisuke, Komiyama, Yuske, Fukumoto, Chonji, Hasegawa, Tomonori, Kawamata, Hitoshi
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01-03-2020
• Subjects: Apoptosis; Cell cycle; Cell Cycle Checkpoints - drug effects; Cell Growth Processes - drug effects; Dental materials; G1 phase; Gene expression; Humans; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; Ligands; Mouth Neoplasms - drug therapy; Mouth Neoplasms - metabolism; Mouth Neoplasms - pathology; mRNA; Oral squamous cell carcinoma; PPAR gamma - biosynthesis; PPAR gamma - genetics; PPAR gamma - metabolism; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck - drug therapy; Squamous Cell Carcinoma of Head and Neck - genetics; Squamous Cell Carcinoma of Head and Neck - metabolism; Squamous Cell Carcinoma of Head and Neck - pathology; Troglitazone; Troglitazone - pharmacology; oral SCC; apoptosis; Peroxisome proliferator-activated receptor γ; troglitazone; G1 arrest
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.14066

We attempted to clarify the role of Peroxisome proliferator-activated receptor γ (PPARγ) and its ligand, troglitazone (TRO) on oral squamous cell carcinoma (SCC).
The expression of PPARγ gene was examined in 47 human oral SCC tissues and two human oral SCC cell lines, CA9-22 and HSC-4. The effects of TRO on the growth and cell-cycle progression of human oral SCC cells were examined.
PPARγ mRNA was detected in 20 of 47 oral SCC tissues and two human oral SCC cells. TRO significantly suppressed the growth of the cells, but did not induce apoptosis. CA9-22 cells treated with TRO showed an increased fraction in the G
phase and decreased fractions in the S and G
-M phases.
TRO did not induce apoptosis in oral SCC cells, but did inhibit the growth of the cells by arresting the cell cycle at G
phase.