Characteristics of extracellular invertase of Saccharomyces cerevisiae in heterologous expression of the suc2 Gene in Solanum tuberosum plants

• Published in: Biology bulletin of the Russian Academy of Sciences Vol. 41; no. 1; pp. 24 - 30
• Main Authors: Deryabin, A. N, Berdichevets, I. N, Burakhanova, E. A, Trunova, T. I
• Format: Journal Article
• Language: English
• Published: Springer-Verlag 2014
• Subjects: apoplast; beta-fructofuranosidase; cell walls; genes; potatoes; proteinases; Saccharomyces cerevisiae; signal peptide; Solanum tuberosum; yeasts
• ISSN: 1062-3590; 1608-3059
• DOI: 10.1134/S1062359014010038

Some properties and activity of extracellular invertase in the Saccharomyces cerevisiae yeasts encoded by the suc2 gene in heterologous expression were described. It was shown that the target suc2 gene is actively expressed in the genome of the transformed potato plants and S. cerevisiae invertase synthesized by this gene is transported into the apoplast due to the signal peptide of the proteinase II inhibitor. This enzyme is present in the apoplast in a soluble form and absorbed into the cell wall.