Hydrodynamic fabrication of polymeric barcoded strips as components for parallel bio-analysis and programmable microactuation

• Published in: Lab on a chip Vol. 5; no. 10; p. 1168
• Main Authors: Kim, SungRak, Oh, HyunJik, Baek, JuYeoul, Kim, HyugHan, Kim, WooSeung, Lee, SangHoon
• Format: Journal Article
• Language: English
• Published: England 01-01-2005
• Subjects: Antigens - chemistry; Catalysis; Dimethylpolysiloxanes; DNA - chemistry; Electrochemistry; Electrophoresis, Microchip - instrumentation; Electrophoresis, Microchip - methods; Enzymes - chemistry; Equipment Design; Hydrogen-Ion Concentration; Lactic Acid - chemistry; Light; Microchemistry; Microfluidic Analytical Techniques - methods; Microfluidics; Microscopy, Fluorescence; Models, Chemical; Oligonucleotide Array Sequence Analysis - methods; Photochemistry; Polymers - chemistry; Protein Array Analysis - methods; Time Factors
• ISSN: 1473-0197
• DOI: 10.1039/b506194f

In this paper, we report a novel technique for the manufacture of polymeric barcoded strips having diverse characteristics such as biocatalyst-based sensing and pH-responsive actuation. The fabrication involves the use of both a microfluidic platform and in-situ photopolymerization. It is expected that this method, which is a very simple, cost-effective, and environment-friendly means for mass production, will facilitate the stable immobilization of diverse biological substances such as enzymes, DNA, and antigens. Therefore, we fabricated an enzyme-immobilized barcoded strip for multiple bio-analysis. On the basis of this technology, we adopted different materials and thereby fabricated programmable microactuation components that are responsive to pH variation.