Resynchronization of follicular wave using long-acting injectable progesterone or estradiol benzoate at 14 days post-timed AI in Bos taurus x Bos indicus beef heifers

• Published in: Theriogenology Vol. 176; pp. 194 - 199
• Main Authors: Vieira, Camila Cupper, Pinto, Hirya Fernandes, Buss, Vanessa, Gonzalez de Freitas, Bruno, Guerreiro, Bruna Martins, Leivas, Fábio Gallas, Pugliesi, Guilherme, Mesquita, Fernando Silveira
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2021
• Subjects: Abortion, Veterinary; Animals; Cattle; Cattle Diseases; Early resynchronization; Estradiol - analogs & derivatives; Estradiol benzoate; Estrus Synchronization; Female; Injectable progesterone; Insemination, Artificial - veterinary; Pregnancy; Progesterone; Estradiol benzoate; Early resynchronization; Injectable progesterone
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2021.09.017

We compared pregnancy rates in beef heifers resynchronized 14 days after the first timed-artificial insemination (TAI) using a P4 intravaginal device associated with either long-acting injectable progesterone (iP4) or estradiol benzoate (EB). Braford and Brangus heifers were submitted to a TAI (D0). On D14, all animals were given a P4 intravaginal device and were randomly divided into two groups, EB (1 mg; n = 339); or iP4 (75 mg; n = 338). On D22, P4 devices were removed, and non-pregnant (NP) heifers were identified by assessing morphological luteolysis with Doppler ultrasonography. The NP heifers had the dominant follicle diameter measured and were submitted to a second TAI on D24. Dominant follicle diameter (mm) on D22 in NP heifers did not differ (P > 0.05) between EB (9.77 ± 0.25) and iP4 (9.92 ± 0.22) groups. No difference was observed between EB and iP4 groups for pregnancy rate on D22 (56.3% vs. 60.1%, respectively), and D40 post-first TAI (49.6% vs. 53.3%, respectively). The rate of potential pregnancy losses from D22 to D40 did not differ between EB (12%, 23/191) and iP4 (11.3%, 23/203) groups. The resynchronization pregnancy rate in the EB group (45.9%, 68/148) was greater (P<0.05) than the iP4 group (31.8%, 43/135). In conclusion, treatment with either 1 mg EB or 75 mg iP4 in combination with P4 device at 14 days after TAI are equally safe for the ongoing pregnancy. The EB treatment can improve the reproductive efficiency, as it resulted in greater resynchronization pregnancy rates than iP4 treatment in beef heifers resynchronized 14 days after TAI.
•Resynchronization with two TAI within 24 days reaches satisfactory pregnancy rates. .•Estradiol benzoate improved resynchronization pregnancy rates.•Estradiol benzoate given at 14 days post-AI did not impact ongoing pregnancy.