Differences in HTLV-I integration patterns between skin lesions and peripheral blood lymphocytes of HTLV-I seropositive patients with cutaneous lymphoproliferative disorders

• Published in: Journal of dermatological science Vol. 4; no. 2; p. 76
• Main Authors: Hamada, T, Setoyama, M, Katahira, Y, Furuno, T, Fujiyoshi, T, Sonoda, S, Tashiro, M
• Format: Journal Article
• Language: English
• Published: Netherlands 01-09-1992
• Subjects: Aged; Female; HTLV-I Antibodies - blood; Human T-lymphotropic virus 1 - genetics; Human T-lymphotropic virus 1 - physiology; Humans; Leukemia-Lymphoma, Adult T-Cell - immunology; Leukemia-Lymphoma, Adult T-Cell - microbiology; Leukemia-Lymphoma, Adult T-Cell - pathology; Lymphocytes - microbiology; Lymphoproliferative Disorders - immunology; Lymphoproliferative Disorders - microbiology; Lymphoproliferative Disorders - pathology; Male; Middle Aged; Polymerase Chain Reaction; Skin - immunology; Skin - microbiology; Skin - pathology; Virus Integration
• ISSN: 0923-1811
• DOI: 10.1016/0923-1811(92)90062-g

We examined HTLV-I integration patterns in nine cases of HTLV-I-seropositive patients with cutaneous lymphoproliferative disorders. The Southern blot on EcoRI digests of DNA revealed a discrete band of HTLV-I provirus (monoclonal integration) in either skin lesions or peripheral blood lymphocytes (PBL). Four cases showed the monoclonal integration of HTLV-I provirus only in skin lesions: one case showed only in PBL and two cases showed in both skin and PBL. The Southern blot on PstI digests of DNA revealed a 2.4 Kb band of the internal construct of HTLV-I provirus (polyclonal integration) in the PBL of EcoRI-negative samples. The difference in HTLV-I integration patterns between skin lesions and PBL in these cases suggests that the monoclonal outgrowth of HTLV-I-infected cells in the skin is causatively associated with the pathogenesis of cutaneous ATL.