Evaluation of PDTC for removing Ni(II) from human serum transferrin in vitro

Pyrrolidine dithiocarbamate (PDTC)can be an oral chelator with pKa=3.300+/-0.002. It behaves as a bidentate ligand at serum pH. The effect of pH on Ni2+-Tf indicated that the maximum adsorption was at pH=7.4. The effective Ni-PDTC binding constant was determined (logk=11.1+/-0.1) for the 1:2 Ni(PDTC...

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Bibliographic Details
Published in:	International journal of artificial organs Vol. 29; no. 8; pp. 809 - 814
Main Authors:	Hooshmand, A, Badiei, A, Fatemi, S J
Format:	Journal Article
Language:	English
Published:	United States 01-08-2006
Subjects:	Adsorption Chelating Agents - pharmacology Humans Hydrogen-Ion Concentration Nickel - antagonists & inhibitors Nickel - blood Protein Binding - drug effects Pyrrolidines - pharmacology Spectrophotometry, Ultraviolet Temperature Thiocarbamates - pharmacology Titrimetry Transferrin - metabolism
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Summary:	Pyrrolidine dithiocarbamate (PDTC)can be an oral chelator with pKa=3.300+/-0.002. It behaves as a bidentate ligand at serum pH. The effect of pH on Ni2+-Tf indicated that the maximum adsorption was at pH=7.4. The effective Ni-PDTC binding constant was determined (logk=11.1+/-0.1) for the 1:2 Ni(PDTC)2 complex using UV-vis spectra. The isosbestic point at 298 indicated that the complexation reaction was done directly (without side reaction). Removal of Ni from transferrin (Tf) was investigated by reverse titration of PDTC at 25 masculineC and pH=7.4 using UV-vis spectra. PDTC is able to remove 25% of Ni from human serum transferrin.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0391-3988 1724-6040
DOI:	10.1177/039139880602900811