Comparison of different sandwich enzyme immunoassays for the quantitation of human apolipoproteins A-I and A-II

Variants of a non-competitive enzyme immunoassay for human apolipoproteins A-I and A-II are described. The method developed is highly sensitive and highly specific. There is a good correlation between the reference technique (electroimmunoassay) and both apolipoprotein A-I and A-II assays (r=0.92)....

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 96; no. 1; p. 115
Main Authors:	Dubois, D Y, Cantraine, F, Malmendier, C L
Format:	Journal Article
Language:	English
Published:	Netherlands 26-01-1987
Subjects:	Apolipoprotein A-I Apolipoprotein A-II Apolipoproteins A - analysis Enzyme-Linked Immunosorbent Assay - methods Humans Immunoenzyme Techniques Polysorbates
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Summary:	Variants of a non-competitive enzyme immunoassay for human apolipoproteins A-I and A-II are described. The method developed is highly sensitive and highly specific. There is a good correlation between the reference technique (electroimmunoassay) and both apolipoprotein A-I and A-II assays (r=0.92). For the apolipoprotein A-I assay, seven simple treatments were studied. Two of these treatments (plasma plus Tween 20 and serum plus apo A-II) greatly increased the precision and the accuracy of the results. For the apo A-II measurement, no treatment of the samples is necessary, but plasma should be preferentially used rather than serum.
ISSN:	0022-1759
DOI:	10.1016/0022-1759(87)90374-7