Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers

• Published in: Czech Journal of Genetics and Plant Breeding Vol. 43; no. 3; pp. 105 - 112
• Main Authors: Yu, C.,Northwest A and F Univ., Yangling (China). Coll. of Agronomy, Leisova, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Kucera, V.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Vyvadilova, M.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Ovesna, J.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Dotlacil, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Hu, S.,Northwest A and F Univ., Yangling (China). Coll. of Agronomy
• Format: Journal Article
• Language: English
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 2007; Czech Academy of Agricultural Sciences
• Subjects: ADN; aflp; Amplified fragment length polymorphism; Brassica; BRASSICA NAPUS; brassica napus l; Breeding; CARACTERISTICAS DE SEMILLAS; CARACTERISTIQUE DES SEMENCES; CHINA; CHINE; Cluster analysis; Clustering; CZECH REPUBLIC; Divergence; DNA; DOMINANT GENES; Fluorescence; FRANCE; FRANCIA; GENE DOMINANT; GENE RECESSIF; GENES DOMINANTES; GENES RECESIVOS; Genetic diversity; GENETIC INHERITANCE; GENETIC MARKERS; GENETIC POLYMORPHISM; GENOTIPOS; GENOTYPE; GENOTYPES; Germplasm; GRAINE DE COLZA; HEREDITE; HERENCIA GENETICA; http://www.fao.org/aos/agrovoc#c_1066; http://www.fao.org/aos/agrovoc#c_1556; http://www.fao.org/aos/agrovoc#c_16022; http://www.fao.org/aos/agrovoc#c_2347; http://www.fao.org/aos/agrovoc#c_2361; http://www.fao.org/aos/agrovoc#c_24030; http://www.fao.org/aos/agrovoc#c_24031; http://www.fao.org/aos/agrovoc#c_25499; http://www.fao.org/aos/agrovoc#c_3081; http://www.fao.org/aos/agrovoc#c_3225; http://www.fao.org/aos/agrovoc#c_33095; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_36509; http://www.fao.org/aos/agrovoc#c_3869; http://www.fao.org/aos/agrovoc#c_6055; http://www.fao.org/aos/agrovoc#c_6470; http://www.fao.org/aos/agrovoc#c_7377; MARCADORES GENETICOS; Markers; MARQUEUR GENETIQUE; METHODE STATISTIQUE; METODOS ESTADISTICOS; Outliers (statistics); PCR; POLAND; POLIMORFISMO GENETICO; POLOGNE; POLONIA; Polymorphism; POLYMORPHISME GENETIQUE; PROCEDENCIA; PROVENANCE; Rape plants; RAPESEED; RECESSIVE GENES; REPUBLICA CHECA; REPUBLIQUE TCHEQUE; SEED CHARACTERISTICS; SEMILLA DE COLZA; STATISTICAL METHODS; yellow seed trait; China
• ISSN: 1212-1975; 1212-1975; 1805-9325
• DOI: 10.17221/2071-cjgpb

The genetic diversity of 35 yellow-seeded Brassica napus accessions originating from China, Czech Republic and Poland was assessed by means of Amplified Fragment Length Polymorphism (AFLP) markers based on multiplex PCR using multi-colour fluorescent-labelled primers. Five brown-seeded accessions originating from China and France were selected as outliers. In total, 632 peaks were generated by AFLP reaction using 18 primer combinations. Only distinctly polymorphic markers among them were scored. In total, 242 polymorphic markers were detected with an average of 13.4 markers per primer combination. The AFLP analysis separated forty studied accessions into Chinese and European groups by UPGMA clustering and Principal Coordinates Analysis (PCA). The grouping of accessions based on the cluster analysis and PCA was generally consistent with known pedigree information and geographic origin. Notable geographical divergence was found between Chinese and European yellow-seeded accessions. This information is useful for yellow-seeded hybrid breeding and encouraging breeders to exchange their germplasm.