Synthesis of multi fluorine containing polyoxometalate sandwich type compound with drug delivery, DNA interaction and protein binding studies

• Published in: Polyhedron Vol. 243; p. 116529
• Main Authors: Khan, Rabbia, Tariq, Muhammad, Shaaban, Ibrahim A., Assiri, Mohammed A., Bhatti, Moazzam H., Muhammad Asif, Hafiz
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-10-2023
• Subjects: Cancer; DNA binding; Drugs; Fluorinated Anhydrides; Polyoxometalates; Drugs; Fluorinated Anhydrides; Polyoxometalates; DNA binding; Cancer
• ISSN: 0277-5387
• DOI: 10.1016/j.poly.2023.116529

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•The synthesis and characterization of Polyoxometalate hybrid from newly synthesized fluorinated anhydride FDA-Di-Tris & FDA@Di-POM.•FDA@Di-POM showed comparatively better results for drug loading and drug release.•UV-visible study of FDA-Di-Tris and FDA@Di-POM with SS-DNA showed hypochromism trend which indicate intercalation mode of attachment.•Protein binding study presented binding constant value (Kb) FDA@Di-POM and FDA-Di-Tris, which are 2.28 × 108 M−1 and 1.55 × 108 M−1, correspondingly.
The combination of polyoxometalates (POMs) and fluorinated anhydride in a variety of modes ofinteractionturned out to be the key for unlocking new directions in the study of biological activities. In this study, two new compounds such as a sandwich type covalently attached fluorinated anhydride [C27H24F6N2O10](FDA-Di-Tris) and a Dawson POM hybrid, [{N(C4H9)4}10 {C22H16F6N2O128P4V6W30·CH3CN}](FDA@Di-POM) were synthesized. These compounds were clearly characterized and their formation was confirmed by spectroscopic(UV–visible, FT-IR, NMR) techniques, elemental analyses and scanning electron microscopy techniques(SEM). FDA-Di-Tris and FDA@Di-POM were evaluated for drug delivery, DNA binding and protein binding. It is found that drug loading efficiency was 82% and 57% for FDA@Di-POM and FDA-Di-Tris,respectively. While drug release for FDA-Di-Tris was found to be lowest (14%) in basic media (pH = 11) and highest (59%) in acidic media (pH = 3) and FDA@Di-POM exhibited approximately similar behavior in both media such as (55%) in basic media (pH = 11) and (49%) in acidic media (pH = 3) respectively. UV–visible study of FDA-Di-Tris and FDA@Di-POM with salmon sperm DNA (SS-DNA) showed hypochromism trend which indicate intercalation mode of attachment and exhibited binding constant value 6.29 × 105M−1 and 8.33 × 105 M−1 for FDA-Di-Tris and FDA@Di-POM, respectively, referring that FDA@Di-POM has more binding affinity than FDA-Di-Tris. Protein binding study presented binding constant value (Kb) FDA@Di-POM and FDA-Di-Tris, which are 2.28 × 108 M−1 and 1.55 108 M−1, correspondingly.