ANTIOXIDANT ACTIVITY AND INHIBITORY EFFECT OF 2,4,4'-TRIHYDROXYCHALCONE ON DIGESTIVE ENZYMES RELATED TO OBESITY

• Published in: Slovenski veterinarski zbornik Vol. 60; no. 25-Suppl
• Main Authors: Alsufiani, Hadeil, Aldhaheri, Ghaynat A., Omar, Ulfat M., Bahdilah, Taraji M., Mansouri, Rasha A.
• Format: Journal Article
• Language: English
• Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 01-01-2023
• Subjects: 2,4,4'-trihydroxychalcone; DPPH radical scavenging activity; ferrous ion chelating activity; reducing power; sucrase; α-amylase
• ISSN: 1580-4003; 2385-8761
• DOI: 10.26873/SVR-1625-2022

The aim of this study was to investigate the antioxidant activity and inhibitory effect of 2,4,4'-trihydroxychalcone on digestive enzymes related to obesity, including sucrase, α-amylase, and lipase. The in vitro antioxidant activities of three concentrations of 2,4,4'-trihydroxychalcone (100, 300, and 500 µg/ml) were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging, reducing power, and ferrous ion chelating assays. Moreover, in vitro inhibition of lipase, α-amylase, and sucrase enzyme activities by 2,4,4'-trihydroxychalcone was determined using a specific assay for each enzyme. 2,4,4'-Trihydroxychalcone has been shown to have antioxidant properties and inhibits sucrase, α-amylase and lipase activities. These findings suggest that 2,4,4′-trihydroxychalcone demonstrated an antioxidant activity and can effectively inhibit the key enzymes related to obesity.