Impact of vte4 and crtRB1 genes on composition of vitamin-E and provitamin-A carotenoids during kernel-stages in sweet corn

• Published in: Journal of food composition and analysis Vol. 105; p. 104264
• Main Authors: Chauhan, Hema S., Chhabra, Rashmi, Rashmi, Tuhin, Muthusamy, Vignesh, Zunjare, Rajkumar U., Mishra, Subhra J., Gain, Nisrita, Mehta, Brijesh K., Singh, Ashok K., Gupta, Hari S., Hossain, Firoz
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-01-2022
• Subjects: Biofortification; Carotene; Gene expression; MAS; Provitamin-A; Sweet corn; Tocopherol; Vitamin-E; proA; df; RNA; QTL; ADP; crtRB1; lcyE; lcyB; UTR; RCBD; vte5; vte4; cDNA; vte3; vte2; hggt; vte1; Provitamin-A; UDP; AC; SDS; PUFA; Carotene; qRT-PCR; Gene expression; psy1; AGPase; InDel; α-T; BCX; γ-T; MAS; BC; mRNA; hppd; ANOVA; γ-TMT; Sweet corn; Biofortification; CD; Vitamin-E; HMG-CoA; MSS; DAP; r; VED; MABB; VAD; sh2; Tocopherol
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2021.104264

•Biofortified sweet corn possessed higher α-tocopherol and provitamin-A.•Vitamin-E attained peak at 24-DAP, while provitamin-A was highest at 20-DAP.•Transcripts of both vte4 and crtRB1 had the highest peak at 24-DAP.•Transcriptsof vte4 increased α-tocopherol, while crtRB1lowered provitamin-A.•First report on effect of vte4 and crtRB1 genes during kernel stages in sweet corn.
Traditional sweet corn is poor in vitamin-E (tocopherols) and provitamin-A carotenoids (β-carotene and β-cryptoxanthin). Here, we analysed six biofortified sweet corn inbreds having mutant γ-tocopherol methyl transferase (vte4) and β-carotene hydroxylase1 (crtRB1) genes, and three traditional sweet corn inbreds with wild-type vte4+ and crtRB1+genes at 20-, 24- and 28-days after pollination (DAP). The biofortified inbreds possessed higher α-tocopherol (α-T: 2.03-folds), γ-tocopherol (γ-T: 1.19-folds), α-/γ-T (1.71-folds), β-carotene (BC: 6.21-folds), β-cryptoxanthin (BCX: 4.14-folds) and total provitamin-A (proA: 5.49-folds) over original inbreds. Vitamin-E attained peak at 24-DAP, while provitamin-A peaked at 20-DAP. Transcripts of vte4 and crtRB1 had the highest peak at 24-DAP. Biofortified inbreds recorded higher transcript level of vte4 (10-folds) and lower level of crtRB1 (8-folds) over vte4+ and crtRB1+. Vte4 transcript was positively associated with α-T, γ-T and α/γ-T. crtRB1 transcript had negative relationship with BC, BCX and proA. This is the first report on dynamic regulation of vte4 and crtRB1 genes during kernel-development among sweet corn.