Comparative Study of Histotripsy Pulse Parameters Used to Inactivate Escherichia coli in Suspension

• Published in: Ultrasound in medicine & biology Vol. 49; no. 12; pp. 2451 - 2458
• Main Authors: Ambekar, Pratik A., Wang, Yak-Nam, Khokhlova, Tatiana, Bruce, Matthew, Leotta, Daniel F., Totten, Stephanie, Maxwell, Adam D., Chan, Keith, Liles, W. Conrad, Dellinger, E. Patchen, Monsky, Wayne, Adedipe, Adeyinka A., Matula, Thomas J.
• Format: Journal Article
• Language: English
• Published: 01-12-2023
• ISSN: 0301-5629; 1879-291X
• DOI: 10.1016/j.ultrasmedbio.2023.08.004

OBJECTIVEBacterial loads can be effectively reduced using cavitation-mediated focused ultrasound, or histotripsy. In this study, gram-negative bacteria (Escherichia coli) in suspension were used as model bacteria to evaluate the effectiveness of two regimens of histotripsy treatments: cavitation histotripsy (CH) and boiling histotripsy (BH). METHODSTen-milliliter volumes of Escherichia coli were treated at different negative focal pressure amplitudes and over time periods up to 40 min. Cavitation activity was characterized with coaxial passive cavitation detection (PCD) and synchronized plane wave B-mode imaging. RESULTSCH treatments exhibited a threshold behavior that was consistent with PCD metrics of cavitation. Above the threshold, bacterial inactivation followed a monotonically increasing log-linear relationship that indicated an exponential inactivation rate. BH exhibited no threshold, but instead followed a different monotonically increasing inactivation rate. Inactivation rates were larger for BH at or below the CH threshold, and larger for CH substantially above the threshold. CH studies performed at different pulse lengths at the same duty cycle had similar inactivation rates, suggesting that at any given pressure amplitude, the "on time" was the most important variable for inactivating E. coli. The maximum inactivation was produced by CH at the highest pressure amplitudes used, leading to a log reduction >4.2 for a 40 min treatment. CONCLUSIONThe results of this study suggest that both CH and BH can be used to inactivate E. coli in suspension, with the optimal regimen depending on the attainable peak negative focal pressure at the target.