Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection

• Published in: Genetics and molecular research Vol. 14; no. 3; pp. 11389 - 11395
• Main Authors: Góis, P B P, Carneiro, M R P, Jain, S, Santos, M I S, Batista, M V A, Cândido, A L
• Format: Journal Article
• Language: English
• Published: Brazil 25-09-2015
• Subjects: Animals; Brazil - epidemiology; Humans; Molecular Typing; Phylogeny; Poultry - microbiology; Random Amplified Polymorphic DNA Technique; Salmonella - classification; Salmonella Infections - epidemiology; Brazil
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.September.25.6

Randomly amplified polymorphic DNA (RAPD) has been widely used for epidemiological and phylogenetic purposes ow-ing to its rapidity and efficiency. The aim of this study was to perform genome typing of Salmonella samples isolated from different sources by RAPD profiling. Thirty-three Salmonella samples from the bacterial collection of the Laboratório de Virologia Comparada, Departamento de Morfologia, Universidade Federal de Sergipe, Brazil, and two standard samples were used. RAPD profiling was conducted using six primers of the Ready-To-Go RAPD system. The amplified products were electro-phoresed on 5% polyacrylamide gel and silver-stained. RAPD analysis resulted in reproducible and stable banding patterns and showed high genetic diversity among the isolated strains. The Primer P1-generated dendrogram showed an epidemiologic relationship between the human and poultry isolated samples, highlighting the usefulness of RAPD for molecular typing and epidemiological studies.