Synthetic dyes decolorization potential of agroindustrial waste-derived thermo-active laccase from Aspergillus species

• Published in: Biocatalysis and agricultural biotechnology Vol. 29; p. 101800
• Main Authors: Omeje, Kingsley O., Nnolim, Nonso E., Ezema, Benjamin O., Ozioko, Juliet N., Eze, Sabinus O.O.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-10-2020
• Subjects: Agro-wastes; Bioremediation; Contamination; Decolorisation; Fungal laccase; Synthetic dye; Fungal laccase; Synthetic dye; Bioremediation; Agro-wastes; Contamination; Decolorisation
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2020.101800

Laccase has become a biocatalyst of interest in biotechnological and industrial sector due to the ability to degrade an array of natural and synthetic toxicants. Consequently, laccase producing fungus was isolated from a soil sample collected from crude oil contaminated site and identified. Laccase production was carried out using medium formulated with agro-wastes, and the produced laccase was partially purified and characterized. Synthetic dyes decolorization potential of the laccase was likewise evaluated. The fungal isolate coded as CSB01 displayed remarkable laccase activity on agar plate. Based on the internal transcribed spacer (ITS) sequence analysis, it was identified as Aspergillus sp. Omeje with GenBank accession number MT476025. Among the agro residues used in the fermentation process, the isolate yielded maximum laccase activity (336 ± 11.31 U/L) when groundnut husk was utilized as carbon source. The time course study showed that Aspergillus sp. Omeje had optimal laccase activity of 920 ± 33.94 U/L at day 9 of fermentation period. Additional, the purified laccase was optimal active at pH and temperature of 7.5 and 50 °C, respectively. The determined Km and Vmax values of the enzyme were 0.14 mM and 0.45 μmol/min, respectively. Furthermore, the degree of synthetic dyes decolorization obtained ranged from 36 ± 11.31% for Basic Violet 2 to 82 ± 12.73% for Vat Blue 5. The findings of the study suggest that the fungal laccase may be useful for industrial and/or bioremediation processes.
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•Fungal isolate with laccase production potential was isolated from oil spill polluted site.•The isolated was identified as Aspergillus sp. Omeje following ITS sequence analysis.•The isolate demonstrated considerable laccase activity in agro-wastes formulated medium.•Purified laccase showed pH and temperature optima of 7.5 and 50 °C, respectively.•The laccase showed considerable potential for the decolorization of synthetic dyes.