Investigation of carbon substrate utilization patterns of three ureolytic bacteria

• Published in: Biocatalysis and agricultural biotechnology Vol. 22; p. 101429
• Main Authors: Mekonnen, Eshetu, Kebede, Ameha, Tafesse, Tekle, Tafesse, Mesfin
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-11-2019
• Subjects: API 50CH; Assimilation; BIOLOG gen III; Carbon substrate; Pattern; Ureolytic; BIOLOG gen III; Assimilation; Pattern; Ureolytic; API 50CH; Carbon substrate
• ISSN: 1878-8181; 1878-8181
• DOI: 10.1016/j.bcab.2019.101429

Bacteria have developed specific substrate utilization profiles which allow them to grow only under specific conditions. They assimilate different organic substrates as carbon and energy sources for their growth and biomass production. In this study the carbon substrate utilization patterns of three bacteria [Bacillus paramycoides (Isolate-3), Citrobacter sedlakii (Isolate-7), and Enterobacter bugadensis (Isolate-11)] were investigated using BIOLOG Gen III microPlate and API strip techniques. These techniques employ various carbon substrates to determine bacterial carbon utilization patterns more quickly, economically, and effectively. Subsequently, biochemical analysis using API 20NE test showed that the three bacterial species were positive for Nitrate reduction, Glucose fermentation, Urease activity, hydrolysis of Esculine, assimilation of N-acetyl-glucosamine, d-maltose and Potassium Gluconate. In addition, the three bacteria assimilated 19 carbon substrates (d-Glucose, D-Sorbitol, Methyl Pyruvate, Dextrin, d-Maltose, d-Fructose, l-Alanine, l-lactic Acid, d-Gluconic Acid, d-Trehalose, d-Cellobiose, Glycerol, Glucuronamide, l-Glutamic Acid, N-Acetyl-d-Glucosamine, b-Gentiobiose, Acetic Acid, l-Serine, and Inosine) of BIOLOG Gen III MicroPlates. On the other hand, they were all unable to assimilate Tween-40, D-Arabitol, d-Lactic Acid, Methyl Ester, d-Fucose, Quinic Acid, and d-Aspartic Acid. Similarly, API 50CH carbohydrate fermentation analysis showed that the three bacteria intensively metabolized d-Ribose, d-Glucose, d-Fructose, N-Acetyl-Glucosamine, Arbutine, Esculine Citrate, d-Maltose, and d-Trehalose. It was also determined that none of the three bacteria could assimilate Erythritol, l-Xylose, D-Adonitol, Methyl-β-d-Xylopyranoside, l-Sorbose, Methyl-α-d-mannopyranoside, Amygdaline, Inuline, d-Melezitose, Amidon, Glycogene, Xylitol, d-turanose, d-Tagatose, d-Fucose, D-Arabitol, L-Arabitol, and Potassium 5-Ceto-Gluconate. The study revealed that Enterobacter bugadensis and Citrobacter sedlakii exhibited an intensive metabolic activity and strong metabolic correlation than Bacillus paramycoides.