Effect of photobiomodulation in an experimental in vitro model of asthma‐Copd overlap

• Published in: Journal of biophotonics Vol. 17; no. 10; pp. e202400124 - n/a
• Main Authors: Guerra, Marina Bertoni, Santana, Kelly Gomes, Momolli, Marcos, Labat, Rodrigo, Chavantes, Maria Cristina, Zammuner, Stella Regina, Júnior, José Antonio Silva, Palma, Renata Kelly, Aimbire, Flavio, Oliveira, Ana Paula Ligeiro
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag GmbH & Co. KGaA 01-10-2024; Wiley Subscription Services, Inc
• Subjects: asthma‐COPD overlap; Chronic obstructive pulmonary disease; Cigarette smoke; cytokines; Dexamethasone; Epithelial cells; Epithelium; House dust; Lasers; low level laser; photobiomodulation; photobiomodulation; asthma‐COPD overlap; low level laser; cytokines
• ISSN: 1864-063X; 1864-0648; 1864-0648
• DOI: 10.1002/jbio.202400124

The objective of the study was to evaluate the effect of photobiomodulation (PBM) with laser on the inflammatory process in an experimental in vitro model of ACO. The groups were: (1) human bronchial epithelial cells (BEAS‐2B); (2) BEAS‐2B cells treated with dexamethasone; (3) BEAS‐2B cells irradiated with laser; (4) BEAS‐2B cells stimulated with cigarette smoke extract (CSE) + House Dust Mite (HDM); (5) BEAS‐2B cells stimulated with CSE + HDM and treated with dexamethasone; (6) BEAS‐2B cells incubated with CSE + HDM and irradiated with laser. After 24 h, cytokines were quantified. There was a reduction in TNF‐α, IL‐1β, IL‐6, IL‐4, IL‐5, IL‐13, IL‐17, IL‐21, IL‐23, and an increase in IL‐10 and IFN‐γ in cells from the laser‐irradiated ACO group compared to only ACO group. With these results, we can suggest that photobiomodulation acts in the modulation of inflammation observed in ACO, and may be a treatment option.
Human bronchial epithelial cells (BEAS‐2B) were stimulated with cigarette smoke extract (CSE) and house dust mite (HDM) for induction of the asthma‐COPD overlap (ACO). There was an increase in the secretion of inflammatory cytokines in the ACO group. Following photobiomodulation therapy (FBM), a reduction in inflammation was demonstrated with a decrease in the concentration of inflammatory cytokines and increase in IL‐10 and IFN‐γ.