Hydrogels with Antioxidant Microparticles Systems Based on Hyaluronic Acid for Regenerative Wound Healing

• Published in: Macromolecular bioscience Vol. 24; no. 10; pp. e2400153 - n/a
• Main Authors: Nacu, Isabella, Ghilan, Alina, Rusu, Alina G., Bercea, Maria, Nita, Loredana E., Vereştiuc, Liliana, Chiriac, Aurica P.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-10-2024
• Subjects: antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Bioactive compounds; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomolecules; Cell Line; Drug delivery; Entrapment; Fourier transforms; Humans; Hyaluronic acid; Hyaluronic Acid - chemistry; Hyaluronic Acid - pharmacology; Hydrogels; Hydrogels - chemical synthesis; Hydrogels - chemistry; Hydrogels - pharmacology; Infrared spectroscopy; Light scattering; Microparticles; Photon correlation spectroscopy; Polymers; Polysaccharides; Quercetin; Quercetin - chemistry; Quercetin - pharmacology; Rheological properties; Rheology; Spectroscopy, Fourier Transform Infrared; Wound healing; Wound Healing - drug effects; quercetin; wound healing; hydrogels; antioxidant activity; hyaluronic acid
• ISSN: 1616-5187; 1616-5195; 1616-5195
• DOI: 10.1002/mabi.202400153

This research focuses on the synthesis of hydrogels exhibiting enhanced antioxidant properties derived from hyaluronic acid (HA) and poly(ethylene brassylate‐co‐squaric acid) (PEBSA), a copolymacrolactone that have the ability to be used in drug delivery applications. Quercetin (Q), a bioflavonoid with strong antioxidant properties, is employed as a bioactive compound. The biomolecule is encapsulated in the polymeric network using different entrapment techniques, including the initial formation of a complex between PEBSA and Q, which is demonstrated through the dynamic light scattering technique. Fourier transform infrared spectroscopy (FT‐IR) and rheological studies confirm the formation of the hydrogels, revealing the occurrence of physical interactions between the synthetic polymer and the polysaccharide. Moreover, the hydrogels demonstrate biocompatible properties after direct contact with the HDFa cell line and antioxidant properties, as revealed by DPPH tests.
This research aims to synthesize a drug‐delivery new hydrogels with increased antioxidant capacity using hyaluronic acid (HA) and poly(ethylene brassylate‐co‐squaric acid) (PEBSA), a copolymacrolactone with a bioactive chemical, quercetin (Q), which is an antioxidant bioflavonoid. Different entrapment methods are used to encapsulate the biomolecule in the polymeric network. The synthetic polymer and polysaccharide interact physically to generate hydrogels. The hydrogels are biocompatible with the HDFA cell line and have antioxidant activity according to DPPH assays.