Multi-Enzyme-Synergetic ultrathin protein nanosheets display high efficient and switch on/off antibacterial activities

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Vol. 416; p. 129082
• Main Authors: Wang, Tingting, Fan, Xiaotong, Li, Ruyu, Xu, Jiayun, Liu, Junqiu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-07-2021
• Subjects: Antibacterial; Interfacial assembly; Multi-enzyme-synergetic; On/off antibacterial activity; On/off antibacterial activity; Interfacial assembly; Multi-enzyme-synergetic; Antibacterial
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2021.129082

Constructing a multi-enzyme synergistic antibacterial system based on the interface assembly strategy, the ultrathin nanosheet structures composed of three enzymes possessed excellent enzyme activities and antibacterial capacities. And the on–off switchable antibacterial activity transformed reversibly which was controlled by protein folding and unfolding.
[Display omitted]
•Ultrathin nanosheets through an interfacial self-assembly strategy are constructed.•Self-assembled multi-enzyme nanosheets are built as tunable antibacterial materials.•The multi-enzyme nanosheets can turn on and off antibacterial activity.•The antibacterial process can be realized for several reversible cycles.
Natural enzymes are expected to be used in biomedical fields due to their extensive antibacterial activity, good biocompatibility and resistance to bacteria. Herein, we constructed a multi-enzyme protein assembly with ultrathin single protein sheet structure by the strategy of interface assembly and developed an efficient and intelligent antibacterial system based on the combination of multi-enzymes. The multi-enzyme protein assembly not only displayed extremely high antibacterial activity, but can also be turned on and off through the denaturant induced folding and unfolding of natural enzymes, and this process can be realized for several reversible cycles. Such an intelligent antibacterial material provides the possibility for its application in biomedical and other fields.