Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus

• Published in: Aquaculture research Vol. 50; no. 9; pp. 2545 - 2562
• Main Authors: Ismail, Mona, Wahdan, Ali, Yusuf, Mohamed, S., Metwally, Elsayed, Mabrok, Mahmoud
• Format: Journal Article
• Language: English
• Published: Oxford Hindawi Limited 01-09-2019
• Subjects: Additives; Antibiotics; Bacterial diseases; bactericidal; Control; Defence mechanisms; Diet; Dietary supplements; Disease resistance; Feeding experiments; Fish; Freshwater fishes; Growth; growth performance; Hematology; Immune response; Immune system; Immunity; Immunology; Innate immunity; Intestine; Intestines; Lysozyme; Marine fishes; Microbiological strains; Oreochromis niloticus; Profiles; Pseudomonas fluorescens; Spleen; Survival; synbiotic Lacto Forte; Tilapia
• ISSN: 1355-557X; 1365-2109
• DOI: 10.1111/are.14212

This study investigated the effects of a synbiotic Lacto Forte on growth performance, haemato‐immunological responses, plasma bactericidal capacity, histological profiles and resistance of Oreochromis niloticus to Pseudomonas fluorescens. The experimental fish were divided into three groups; two of them (T1 and T2) were given Lacto Forte incorporated diets at increasing level (0.7 and 1.5 g/kg, respectively) and an additive‐free basal diet served as the control (T0) for 30 and 45 days. At the end of the feeding trial, a challenge was performed using a virulent strain of P. fluorescens and mortalities were recorded over an additional 14‐days period. The results showed that Lacto Forte has a pronounced effect on haematological and growth performance parameters at 1.5 g/kg rather than 0.5 g/kg (p < 0.05). Plasma lysozyme, proteases, antiproteases and bactericidal capacity were significantly enhanced (p < 0.05) in Lacto Forte‐treated groups, particularly after 45 days of supplementation. Long‐term supplementation with Lacto Forte (1.5 g/kg) induced degenerative changes in the liver, spleen and intestine. Additionally, a significant increase (p < 0.05) in survival rates were found in Lacto Forte‐treated groups compared to the control one 14 days post‐challenge. As a result, Lacto Forte fortifies tilapia immune response and can be used as a surrogate for antibiotics to control P. fluorescens.